Proteomics

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Stage II of Chronic Kidney Disease - a Tipping Point?


ABSTRACT: Background Chronic kidney disease (CKD) is the progressive loss of renal function. Although advances have been made in understanding the progression of CKD, key molecular events in complex pathophysiological mechanisms that mark each stage of renal failure remain largely unknown. Changes in plasma protein profiles in different disease stages are important for identification of early diagnostic markers and potential therapeutic targets. The goal of this study was to determine the molecular profile of each CKD stage (from 1 to 5), aiming to specifically point out markedly expressed or downregulated proteins. Methods We performed a cross-sectional shotgun-proteomic study of plasma proteome across CKD stages and compared them to healthy controls. After sample pooling and heparin-column purification we analysed proteomes from healthy to CKD stage 1 through 5 participants’ plasma by liquid-chromatography/mass-spectrometry. Results We identified 298 proteins in all study groups, as well as several outliers. Our results indicate that key events, that may later affect the course of disease progression and the overall pathophysiological background, are most pronounced in CKD stage 2, with an emphasis on inflammation, lipoprotein metabolism, angiogenesis and tissue regeneration. Conclusions Our data is consistent with multiple previous studies in CKD research, but also uncovers previously unknown CKD mediators and brings forth several novel potential protein-candidates which might have a significant impact on the development of diagnostic and therapeutic strategies for CKD. We hypothesize that CKD stage 2 is the tipping point in disease progression and a suitable point in disease course for the development of therapeutic solutions.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

DISEASE(S): Chronic Kidney Disease

SUBMITTER: Ruđer Novak  

LAB HEAD: Lovorka Grgurević

PROVIDER: PXD033427 | Pride | 2022-08-12

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
MM_20220127_MEF_01A.raw Raw
MM_20220127_MEF_01B.raw Raw
MM_20220127_MEF_02A.raw Raw
MM_20220127_MEF_02B.raw Raw
MM_20220127_MEF_03A.raw Raw
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Publications

Stage II of Chronic Kidney Disease-A Tipping Point in Disease Progression?

Grgurevic Lovorka L   Novak Rudjer R   Salai Grgur G   Hrkac Stela S   Mocibob Marko M   Vojtusek Ivana Kovacevic IK   Laganovic Mario M  

Biomedicines 20220627 7


Chronic kidney disease (CKD) is the progressive loss of renal function. Although advances have been made in understanding the progression of CKD, key molecular events in complex pathophysiological mechanisms that mark each stage of renal failure remain largely unknown. Changes in plasma protein profiles in different disease stages are important for identification of early diagnostic markers and potential therapeutic targets. The goal of this study was to determine the molecular profile of each C  ...[more]

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