Proteomics

Dataset Information

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Dexamethasone is a regulator of human testicular peritubular cells / Proteome 72 h


ABSTRACT: The functions of human testicular peritubular cells (HTPCs), which form a small compartment located between the seminiferous epithelium and the interstitials areas of the testis, go beyond intratesticular sperm transport and include immunological roles. The expression of the glucocorticoid receptor (GR/NR3C1) by these cells indicates that they are a target of glucocorticoids (GCs), yet detailed insights into consequences of GC actions are unknown. To address this point, we studied cultured HTPCs, which serve as a window into the human testis. We used a cytokine profiler assay to screen for changes in secreted cytokines upon dexamethasone (Dex) treatment and employed qPCR and ELISA studies to verify the results. Dex, used in a therapeutic concentration, decreased proinflammatory factors, including IL6, IL8, MCP1 and CXCL1 within 24 - 48 h. An siRNA-mediated knockdown of GR reduced these actions. Exposure to Dex resulted in reduced GR levels, implying that exposure to Dex could also reduce anti-inflammatory actions of Dex. To evaluate the influence of Dex on HTPCs further, we performed a proteomic analysis of cellular components and secreted proteins. Strong responses were detectable after 24 h (31 significantly altered cellular proteins) and more pronounced ones after 72 h (30 less abundant and 42 more abundant cellular proteins). Dex also altered the composition of the secretome (33 proteins decreased, 13 increased) after 72 h. Among the regulated proteins were ECM- and basement membrane components (e.g. FBLN2, COL1A2, COL3A1), as well as PTX3 and StAR. These results reveal that Dex via GR exerts profound actions in HTPCs. If transferrable to the human testis, changes specifically in ECM and the immunological state of the testis may result in men upon treatment with Dex for medical reasons. -Proteome Data Subset 72 h-

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Peritubular Myoid Cell

SUBMITTER: Youli Konstantinovitch Stepanov  

LAB HEAD: Dr. Thomas Fröhlich

PROVIDER: PXD033534 | Pride | 2023-03-11

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
02.10.19_c1.raw Raw
02.10.19_c2.raw Raw
02.10.19_c3.raw Raw
02.10.19_dex1.raw Raw
02.10.19_dex2.raw Raw
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Publications

Profound Effects of Dexamethasone on the Immunological State, Synthesis and Secretion Capacity of Human Testicular Peritubular Cells.

Stepanov Youli Konstantinovitch YK   Speidel Jan Dominik JD   Herrmann Carola C   Schmid Nina N   Behr Rüdiger R   Köhn Frank-Michael FM   Stöckl Jan Bernd JB   Pickl Ulrich U   Trottmann Matthias M   Fröhlich Thomas T   Mayerhofer Artur A   Welter Harald H  

Cells 20221009 19


The functions of human testicular peritubular cells (HTPCs), forming a small compartment located between the seminiferous epithelium and the interstitial areas of the testis, are not fully known but go beyond intratesticular sperm transport and include immunological roles. The expression of the glucocorticoid receptor (GR) indicates that they may be regulated by glucocorticoids (GCs). Herein, we studied the consequences of the GC dexamethasone (Dex) in cultured HTPCs, which serves as a unique wi  ...[more]

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