Dataset Information

Proteome of arsenite in P. pseudoalcaligenes CECT 5344

ABSTRACT: Quantitative proteomic analysis of P. pseudoalcaligenes CECT 5344 in the presence of arsenite (As(III)) with ammonium and cyanide as nitrogen sources

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Bacteria Pseudomonas Pseudoalcaligenes Cect 5344

SUBMITTER: Alfonso Olaya Abril

LAB HEAD: Conrado Moreno Vivián

PROVIDER: PXD033539 | Pride | 2023-05-10

REPOSITORIES: Pride

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			Action	DRS
	VictorLuque_sol324-8-20_10_09092020.raw	Raw
	VictorLuque_sol324-8-20_11_09092020.raw	Raw
	VictorLuque_sol324-8-20_12_09092020.raw	Raw
	VictorLuque_sol324-8-20_1_09092020.raw	Raw
	VictorLuque_sol324-8-20_2_09092020.raw	Raw

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Publications

Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by Pseudomonas pseudoalcaligenes CECT 5344.

Biełło Karolina A KA Cabello Purificación P Rodríguez-Caballero Gema G Sáez Lara P LP Luque-Almagro Víctor M VM Roldán María Dolores MD Olaya-Abril Alfonso A Moreno-Vivián Conrado C

International journal of molecular sciences 20230413 8

Wastewater from mining and other industries usually contains arsenic and cyanide, two highly toxic pollutants, thereby creating the need to develop bioremediation strategies. Here, molecular mechanisms triggered by the simultaneous presence of cyanide and arsenite were analyzed by quantitative proteomics, complemented with qRT-PCR analysis and determination of analytes in the cyanide-assimilating bacterium Pseudomonas pseudoalcaligenes CECT 5344. Several proteins encoded by two ars ...[more]

PMID: 37108394

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Project description:One of the central goals of evolutionary biology is to explain and predict the molecular basis of adaptive evolution. We studied the evolution of genetic networks in Saccharomyces cerevisiae (budding yeast) populations propagated for more than 200 generations in different nitrogen-limiting conditions. We find that rapid adaptive evolution in nitrogen-poor environments is dominated by the de novo generation and selection of copy number variants (CNVs), a large fraction of which contain genes encoding specific nitrogen transporters including PUT4, DUR3 and DAL4. The large fitness increases associated with these alleles limits the genetic heterogeneity of adapting populations even in environments with multiple nitrogen sources. Complete identification of acquired point mutations, in individual lineages and entire populations, identified heterogeneity at the level of genetic loci but common themes at the level of functional modules, including genes controlling phosphatidylinositol-3-phosphate metabolism and vacuole biogenesis. Adaptive strategies shared with other nutrient-limited environments point to selection of genetic variation in the TORC1 and Ras/PKA signaling pathways as a general mechanism underlying improved growth in nutrient-limited environments. Within a single population we observed the repeated independent selection of a multi-locus genotype, comprised of the functionally related genes GAT1, MEP2 and LST4. By studying the fitness of individual alleles, and their combination, as well as the evolutionary history of the evolving population, we find that the order in which these mutations are acquired is constrained by epistasis. The identification of repeatedly selected variation at functionally related loci that interact epistatically suggests that gene network polymorphisms (GNPs) may be a frequent outcome of adaptive evolution. Our results provide insight into the mechanistic basis by which cells adapt to nutrient-limited environments and suggest that knowledge of the selective environment and the regulatory mechanisms important for growth and survival in that environment greatly increases the predictability of adaptive evolution. mRNA from each evolved clone or from the ancestral strain growing in the specificied nitrogen-limited condition was co-hybridized with mRNA from the ancestral strain grown in ammonium limited media

Dataset Information

Proteome of arsenite in P. pseudoalcaligenes CECT 5344

Dataset's files

Publications

Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by <i>Pseudomonas pseudoalcaligenes</i> CECT 5344.

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