Proteomics

Dataset Information

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TET1 interactome by SILAC IP-MS in mouse ESCs


ABSTRACT: TET1 maintains hypomethylation at bivalent promoters through its catalytic activity in embryonic stem cells (ESCs). However, whether and how TET1 exerts catalytic activity-independent functions in regulating bivalent genes is not well understood. Therefore, we mapped the TET1 interactome in mouse ESCs using a SILAC IP-MS proteomics approach.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Embryonic Stem Cell

SUBMITTER: Xin Huang  

LAB HEAD: Jianlong Wang

PROVIDER: PXD033587 | Pride | 2022-06-10

REPOSITORIES: Pride

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Publications

A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency.

Huang Xin X   Bashkenova Nazym N   Hong Yantao Y   Lyu Cong C   Guallar Diana D   Hu Zhe Z   Malik Vikas V   Li Dan D   Wang Hailin H   Shen Xiaohua X   Zhou Hongwei H   Wang Jianlong J  

Cell reports 20220601 10


TET1 maintains hypomethylation at bivalent promoters through its catalytic activity in embryonic stem cells (ESCs). However, TET1 catalytic activity-independent function in regulating bivalent genes is not well understood. Using a proteomics approach, we map the TET1 interactome in ESCs and identify PSPC1 as a TET1 partner. Genome-wide location analysis reveals that PSPC1 functionally associates with TET1 and Polycomb repressive complex-2 (PRC2). We establish that PSPC1 and TET1 repress, and the  ...[more]

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