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METATAXONOMIC AND METAPROTEOMIC PROFILING OF THE ORAL MICROBIOME IN ORAL LICHEN PLANUS

ABSTRACT: Background: A growing body of evidence demonstrates a different bacterial composition in the oral cavity of patients with oral lichen planus (OLP). Patients and methods: Buccal swab samples were collected from affected and non-affected sites of six patients with reticular OLP and the healthy oral mucosa of six control subjects. 16S rRNA MiSeq sequencing and mass spectrometry-based proteomics and were utilised to identify the metataxonomic and metaproteomic profiles of the oral microbiome in both groups. Results: The most abundant species in the three subgroups were Streptococcus oralis and Pseudomonas aeruginosa, accounting for up to 70% of the total population. A Canonical Correspondence Analysis showed differential clustering of samples from the healthy and OLP groups. Three species (Veillonella parvula, Actinomyces sp, and Lactococcus lactis) were significantly over-represented in the control group and one (Granulicatella elegans) in patients with OLP. The metaproteomic data revealed that several G. haemolysans-belonging peptidases and other proteins with inflammatory and virulence potential were found present in OLP lesions only. Conclusion: Our data suggest that several bacterial species and peptides are associated with OLP. Future studies with larger cohorts should be conducted to determine their role in the aetiology of OLP and evaluate their potential as disease biomarkers.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Streptococcus Oralis

DISEASE(S): Lichen Planus

SUBMITTER: Evelin Berger

LAB HEAD: Maria Bankvall

PROVIDER: PXD033687 | Pride | 2025-04-03

REPOSITORIES: Pride

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			Action	DRS
	3042_TargetDecoy_DoNotMerge_QEHF_181122_39.pdResult	Other
	QEHF_181122_100-_1_.msf	Msf
	QEHF_181122_100.raw	Raw
	QEHF_181122_39.msf	Msf
	QEHF_181122_39.raw	Raw

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