Dataset Information

GT-stabiligase N-terminomics technology for capturing proteolytic modifications on human cell surfaces

ABSTRACT: Here we introduce a stabiligase technology that directly identifies neo-proteolytic epitopeson the cell surface of human cells using a glycan-tethered, engineered peptide ligase.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Epithelial Cell

DISEASE(S): Breast Cancer

SUBMITTER: Kaitlin Schaefer

LAB HEAD: Kaitlin Schaefer

PROVIDER: PXD033811 | Pride | 2023-06-23

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	201106_200ng_OGA1S_293T_Slot2-50_1_473.d.refined.mgf	Mgf
	201106_200ng_OGA1S_293T_Slot2-50_1_473.d.zip	Other
	201106_200ng_PEG7A1S_293T_Slot2-49_1_472.d.refined.mgf	Mgf
	201106_200ng_PEG7A1S_293T_Slot2-49_1_472.d.zip	Other
	201216_hHeL_Slot2-52_1_655.d.refined.mgf	Mgf

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Publications

Direct Identification of Proteolytic Cleavages on Living Cells Using a Glycan-Tethered Peptide Ligase.

Schaefer Kaitlin K Lui Irene I Byrnes James R JR Kang Emily E Zhou Jie J Weeks Amy M AM Wells James A JA

ACS central science 20221011 10

Proteolytic cleavage of cell surface proteins triggers critical processes including cell-cell interactions, receptor activation, and shedding of signaling proteins. Consequently, dysregulated extracellular proteases contribute to malignant cell phenotypes including most cancers. To understand these effects, methods are needed that identify proteolyzed membrane proteins within diverse cellular contexts. Herein we report a proteomic approach, called cell surface N-terminomics, to broadly identify ...[more]

PMID: 36313159

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