Dataset Information

Identification of potential visceral pain biomarkers in colon exudates from mice with experimental colitis: an exploratory in vitro study

ABSTRACT: Chronic visceral pain (CVP) is extremely difficult to diagnose, and available analgesic treatment options are quite limited. Identifying the proteins secreted from the colonic nociceptors, or their neighbor cells within the tube walls, in the context of disorders that course with visceral pain, might be useful to decipher the mechanism involved in the establishment of CVP. Addressing this question in patients with gastrointestinal disorders entails multiple difficulties, as there is not a clear classification of disease severity, and colonic secretion is not easy to manage.We propose using of a murine model of colitis to identify new algesic molecules and pathways that could be explored as pain biomarkers or analgesia targets.Descending colons from naïve and colitis mice with visceral hyperalgesia were excised and maintained ex vivo. The proteins secreted in the perfusion fluid before and during acute noxious distension were evaluated using high-resolution mass spectrometry (MS). Haptoglobin (Hp), PZD and LIM domain protein 3 (Pdlim3), NADP-dependent malic enzyme (Me1), and Apolipoprotein A-I (Apoa1) were increased during visceral insult, whilst Triosephosphate isomerase (Tpi1), Glucose-6-phosphate isomerase (Gpi1), Alpha-enolase (Eno1), and Isoform 2 of Tropomyosin alpha-1 chain (Tpm1) were decreased.Most identified proteins have been described in the context of different chronic pain conditions and, according to gene ontology analysis, they are also involved in diverse biological processes of relevance.Thus, animal models that mimic human conditions in combination with unbiased omics approaches will ultimately help to identify new pathophysiological mechanisms underlying pain that might be useful in diagnosing and treating pain.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Urine, Colon

SUBMITTER: Casimiro Cardenas

LAB HEAD: Carolina Laura Roza

PROVIDER: PXD033902 | Pride | 2023-01-11

REPOSITORIES: Pride

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			Action	DRS
	210427_TMT2plex-1-TR1.raw	Raw
	210427_TMT2plex-1-TR2.raw	Raw
	210427_TMT2plex-1-TR3.raw	Raw
	210427_TMT2plex-2-TR1.raw	Raw
	210427_TMT2plex-2-TR2.raw	Raw

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Publications

Multisociety Consensus Quality Improvement Revised Consensus Statement for Endovascular Therapy of Acute Ischemic Stroke.

Sacks David D Baxter Blaise B Campbell Bruce C V BCV Carpenter Jeffrey S JS Cognard Christophe C Dippel Diederik D Eesa Muneer M Fischer Urs U Hausegger Klaus K Hirsch Joshua A JA Shazam Hussain Muhammad M Jansen Olav O Jayaraman Mahesh V MV Khalessi Alexander A AA Kluck Bryan W BW Lavine Sean S Meyers Philip M PM Ramee Stephen S Rüfenacht Daniel A DA Schirmer Clemens M CM Vorwerk Dierk D

International journal of stroke : official journal of the International Stroke Society 20180522 6

PMID: 29786478

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