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HPLC-MS datasets of the protein mix immunoprecipitated by SIRT7 antibody in HaCaT cells

ABSTRACT: We conducted HPLC-MS analysis of the protein mix immunoprecipitated by SIRT7 antibody under non-IR and 8 Gy X-ray irradiation to identify the interacting proteins of SIRT7 in HaCaT cells.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Keratinocyte, Skin

SUBMITTER: Xingyuan Liu

LAB HEAD: Yi Jin

PROVIDER: PXD033924 | Pride | 2023-03-10

REPOSITORIES: Pride

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			Action	DRS
	LXT-1.msf	Msf
	LXT-1.msfView	Msf
	LXT-1.pdResult	Other
	LXT-1.pdResultView	Other
	LXT-1.raw	Raw

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Publications

Resveratrol rescues cutaneous radiation-induced DNA damage via a novel AMPK/SIRT7/HMGB1 regulatory axis.

Jin Yi Y Liu Xingyuan X Liang Xiaoting X Liu Jiabin J Liu Jieyu J Han Zonglin Z Lu Qianxin Q Wang Ke K Meng Bingyao B Zhang Chunting C Xu Minna M Guan Jian J Ma Li L Zhou Liang L

Cell death & disease 20230101 10

Cutaneous radiation injury (CRI) interrupts the scheduled process of radiotherapy and even compromises the life quality of patients. However, the current clinical options for alleviating CRI are relatively limited. Resveratrol (RSV) has been shown to be a promising protective agent against CRI; yet the mechanisms of RSV enhancing radioresistance were not fully elucidated and limited its clinical application. In this study, we demonstrate RSV promotes cutaneous radioresistance mainly through SIRT ...[more]

PMID: 36587031

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Gene expression profile at single cell level of HaCaT cells exposed to ionizing radiation

Project description:HaCaT cell line is a kind of keratinocyte from human skin. We used single cell RNA sequencing (scRNA-seq) to analyze the influnce of fractionated 2 Gy or single 20 Gy irradiation on HaCaT cells.

2022-12-31 | GSE210170 | GEO

SIRT7 suppresses tumorigenesis by attenuating MYC activity

Project description:SIRT7 is a member of the mammalian sirtuin family of NAD+ dependent deacylases, and interacts with RNA polymerase I and UBF to regulate rDNA transcription. Various studies in mammalian cells and human clinical data have linked SIRT7 to cancer. However studies differ as to whether SIRT7 is oncogenic or tumor suppressive. Here we analyzed SIRT7 knockout mice and found SIRT7 deficiency caused sub-Mendelian birth numbers and a reduction in body size. Moreover, at 18 month of age, roughly 60 % of the SIRT7 knockout mice develop hepatocellular carcinoma (HCC), in many cases leading to suspected metastasis. Several HCC associated genes were up-regulated in livers of mice as young as 6 months of age, particularly targets of the proto-oncogene, c- MYC. Indeed SIRT7 interacts with MYC at endogenous protein levels and also represses MYC activity. Our findings thus show that SIRT7 acts as a tumor suppressor in vivo, and may suggest novel strategies to treat liver cancer. The mRNAs from 3 replicates of mouse Wildtype liver compared to 3 replicates of mouse liver lacking SIRT7

2014-08-20 | E-GEOD-50076 | biostudies-arrayexpress

cDNA array 6800 genes (NIB) - HaCaT J10 24h - 0.5&2 Gy

Project description:Human HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye-swap hybridization procedure 24 h after irradiation. Keywords = human keratinocytes, gamma irrdiation Keywords: dose response

2003-03-26 | GSE356 | GEO

cDNA array 6800 genes (NIB) - HaCaT J10 3h - 0.5&2 Gy

Project description:Huamn HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye-swap hybridization procedure 3 h after irradiation. Keywords = Human keratinocytes, gamma irradiation Keywords: dose response

2003-03-13 | GSE338 | GEO

cDNA array 2304 genes (OSPE) - HaCaT J10 3h - 0.5&2 Gy

Project description:Human HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye swap hybridization procedure 3 h after irradiation. Keywords = Human keratinocytes, gamma irradiation Keywords: dose response

2003-03-11 | GSE337 | GEO

cDNA array 2304 genes (OSPE) - HaCaT J10 3h - 0.5&2 Gy

Project description:Human HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye swap hybridization procedure 3 h after irradiation. Keywords = Human keratinocytes, gamma irradiation

2010-06-05 | E-GEOD-337 | biostudies-arrayexpress

cDNA array 6800 genes (NIB) - HaCaT J10 24h - 0.5&2 Gy

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SIRT7 is a histone H3 lysine 18 deacetylase that regulates oncogenic transformation

Project description:Sirtuin proteins regulate diverse cellular pathways that influence genomic stability, metabolism, and ageing. SIRT7 is a mammalian sirtuin whose biochemical activity, molecular targets, and physiologic functions have been unclear. Here we show that SIRT7 is an NAD+-dependent, histone H3 acetyl-lysine 18 (H3K18Ac) deacetylase that stabilizes the transformed state of cancer cells. Genome-wide binding studies reveal that SIRT7 binds to promoters of a specific set of gene targets, where it deacetylates H3K18Ac and promotes transcriptional repression. The spectrum of SIRT7 target genes is defined in part by interaction of SIRT7 with the cancer-related ETS transcription factor ELK4, and comprises numerous genes with links to tumour suppression. Notably, selective hypoacetylation of H3K18Ac has recently been linked to oncogenic transformation, and in patients, is associated with aggressive tumour phenotypes and poor prognosis. We find that deacetylation of H3K18Ac by SIRT7 is necessary for maintaining essential features of human cancer cells including anchorage independent growth and escape from contact inhibition. Moreover, SIRT7 is necessary for a global hypoacetylation of H3K18Ac associated with cellular transformation by viral oncoproteins. Finally, SIRT7 depletion markedly reduces the tumourigenicity of human cancer cell xenografts in mice. Together, our work establishes SIRT7 as the first known site-specific H3K18Ac deacetylase and demonstrates a pivotal role for SIRT7 in chromatin regulation, cellular transformation programs, and tumour formation in vivo. SIRT7 ChIP-seq was performed in K562 cell lines under normal conditions. Two replicates were performed for both the SIRT7 ChIP as well as input control.

2012-05-06 | E-GEOD-28149 | biostudies-arrayexpress

cDNA array 6800 genes (NIB) - HaCaT J10 3h - 0.5&2 Gy

2003-03-13 | E-GEOD-338 | biostudies-arrayexpress

SIRT7 safeguards genome stability and suppresses colorectal tumorigenesis

Project description:SIRT7 is a member of the mammalian sirtuin family and functions as a NAD+-dependent deacylase. Studies in culture cells and human clinical data have implicated the role of SIRT7 in tumorigenesis. However, controversies were raised as to whether SIRT7 is oncogenic or tumor suppressive. Here we show that SIRT7 deficiency led to aneuploidy and aging-phenotypes, including senescence and nucleolin expansion. SIRT7 knockout mice were susceptible to DSS-induced colitis and alcohol-derived DNA damage, in advance led to intestinal epithelial barrier disruption. Devoid of SIRT7 aggravated the susceptibility of colorectal cancer incidence in APCMin/+ mouse model with further dysregulated Wnt signaling. Our findings indicated a tumor suppressive role of SIRT7 in vivo, novel strategies design for activating SIRT7 in treating colon cancer may be reappraised.

2020-09-16 | GSE138289 | GEO

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