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Identification of site-specific incorporation of Kacyl/K*acyl in full-length histones in E. coli and mammalian cells

ABSTRACT: With the evolution of the PylRS system, we identified the PylRS variants for incorporation of all synthesized Kacyl and Kacyl* in the full-length H3 protein both in E. coli and mammalian cells, which covered the tail domain (eg. K23, K27, K56) as well as the globular domain that are difficult for chemical synthesis (eg. K64, K79, K122).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Early Embryonic Cell

DISEASE(S): Disease Free

SUBMITTER: Fangfei Qin

LAB HEAD: Peng R. Chen

PROVIDER: PXD029504 | Pride | 2023-03-06

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	E.coli-H3K_23pr.raw	Raw
	E.coli-H3K_23pr.xlsx	Xlsx
	E.coli-H3K_27ac.raw	Raw
	E.coli-H3K_27ac.xlsx	Xlsx
	E.coli-H3K_27bhb.raw	Raw

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Publications

Linking chromatin acylation mark-defined proteome and genome in living cells.

Qin Fangfei F Li Boyuan B Wang Hui H Ma Sihui S Li Jiaofeng J Liu Shanglin S Kong Linghao L Zheng Huangtao H Zhu Rongfeng R Han Yu Y Yang Mingdong M Li Kai K Ji Xiong X Chen Peng R PR

Cell 20230301 5

A generalizable strategy with programmable site specificity for in situ profiling of histone modifications on unperturbed chromatin remains highly desirable but challenging. We herein developed a single-site-resolved multi-omics (SiTomics) strategy for systematic mapping of dynamic modifications and subsequent profiling of chromatinized proteome and genome defined by specific chromatin acylations in living cells. By leveraging the genetic code expansion strategy, our SiTomics toolkit revealed di ...[more]

PMID: 36868209

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