Dataset Information

Proteomics investigation of Nucleostemin role in Nucleo-phosmin mutated OCI-AML 3 cell line

ABSTRACT: Nucleostemin (NS; product of the GNL3 gene) is a nucleolar/nucleoplasm shuttling GTPase whose levels are high in stem cells while rapidly decreasing upon differentiation. NS levels are also high in several solid and hematological neoplasms, including acute myeloid leukemia (AML). While a role in telomere maintenance, response to stress stimuli and in favoring DNA repair has been proposed in solid cancers, little or no information is available as to the role of nucleostemin in AML. Here we investigate this issue with a proteomics approach. We use as a model system the OCI-AML 3 cell line harboring a heterozygous mutation at the NPM1 gene, which is the most frequent driver mutation in AML (approximately 30% of total AML cases). We show that NS is highly expressed in this cell line but, contrary to what previously shown in other cancers, its presence is dispensable for cell growth and viability. However, proteomics analysis of OCI-AML 3 cell line before and after nu-cleostemin (NS) silencing showed several effects in different biological functions as highlighted by ingenuity pathway analysis (IPA). In particular, we report an effect in down-regulating DNA repair through homologous recombination and we confirmed higher DNA damage rate in OCI-AML 3 cells when NS is depleted, which considerably increases upon stress induced by the topoisomerase II inhibitor etoposide.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell

DISEASE(S): Acute Leukemia

SUBMITTER: Maria Concetta Cufaro

LAB HEAD: Piero Del Boccio

PROVIDER: PXD034012 | Pride | 2022-08-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	14112020_OCI3_SCR_01_2uL.raw	Raw
	14112020_OCI3_SCR_02_2uL.raw	Raw
	14112020_OCI3_SCR_03_2uL.raw	Raw
	14112020_OCI3_SINS_01_2uL.raw	Raw
	14112020_OCI3_SINS_02_2uL.raw	Raw

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Publications

Proteomic Investigation of the Role of Nucleostemin in Nucleophosmin-Mutated OCI-AML 3 Cell Line.

Cela Ilaria I Cufaro Maria Concetta MC Fucito Maurine M Pieragostino Damiana D Lanuti Paola P Sallese Michele M Del Boccio Piero P Di Matteo Adele A Allocati Nerino N De Laurenzi Vincenzo V Federici Luca L

International journal of molecular sciences 20220711 14

Nucleostemin (NS; a product of the <i>GNL3</i> gene) is a nucleolar-nucleoplasm shuttling GTPase whose levels are high in stem cells and rapidly decrease upon differentiation. NS levels are also high in several solid and hematological neoplasms, including acute myeloid leukaemia (AML). While a role in telomere maintenance, response to stress stimuli and favoring DNA repair has been proposed in solid cancers, little or no information is available as to the role of nucleostemin in AML. Here, we in ...[more]

PMID: 35886999

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