Dataset Information

Proteomics of Prevotella brevis GA33 and Prevotella ruminicola 23

ABSTRACT: We performed shotgun proteomics on the bacteria Prevotella brevis GA33 and Prevotella ruminicola 23. We did this for two types of samples (cell extract and cell membrane) and using two methods (data-dependent and data-independent acquisition).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Acyrthosiphon Pisum (pea Aphid)

TISSUE(S): Cell Culture

SUBMITTER: Timothy Hackmann

LAB HEAD: Timothy John Hackmann

PROVIDER: PXD034119 | Pride | 2023-10-24

REPOSITORIES: Pride

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			Action	DRS
	Prevotella_brevis_GA33_Cell_extract_DDA_Replicate_1.raw	Raw
	Prevotella_brevis_GA33_Cell_extract_DDA_Replicate_1.xml	Xml
	Prevotella_brevis_GA33_Cell_extract_DDA_Replicate_2.raw	Raw
	Prevotella_brevis_GA33_Cell_extract_DDA_Replicate_2.xml	Xml
	Prevotella_brevis_GA33_Cell_membrane_DDA_Replicate_1.raw	Raw

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Publications

The oxidoreductase activity of Rnf balances redox cofactors during fermentation of glucose to propionate in Prevotella.

Zhang Bo B Lingga Christopher C De Groot Hannah H Hackmann Timothy J TJ

Scientific reports 20230930 1

Propionate is a microbial metabolite formed in the gastrointestinal tract, and it affects host physiology as a source of energy and signaling molecule. Despite the importance of propionate, the biochemical pathways responsible for its formation are not clear in all microbes. For the succinate pathway used during fermentation, a key enzyme appears to be missing-one that oxidizes ferredoxin and reduces NAD. Here we show that Rnf [ferredoxin-NAD<sup>+</sup> oxidoreductase (Na<sup>+</sup>-transporti ...[more]

PMID: 37777597

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