Dataset Information

Oenothera effect on senescent NDHF

ABSTRACT: Accumulation of senescent dermal fibroblasts drives skin aging, impairing the integrity of the extracellular matrix (ECM) and the function of neighbouring cells. One of strategies to manipulate cell senescence include the reactivation of proliferation. Here, a data independent mass spectrometry based proteomic approach has been used to evaluate the effect of an hydrophilic Oenothera biennis cell culture extract (ObHEx) on senescent human dermal fibroblasts. It has been shown that the extract was able to affect the levels of 18 proteins which cluster together and point to mitosis pathways. Indeed the treatment with ObHEx increase the expression of CDK1, replicative helicase complex (MCM2, MCM3, MCM4, MCM5, MCM6, MCM7), condensin I complex (NCAPD2, NCAPH, NCAPG, SMC4, SMC2) and other proteins related to kinetochore (KNTC1, NUF2, TRIP13). This strongly suggests that ObHEx could restore the proliferation of senescent fibroblasts.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Cerina Chhuon

LAB HEAD: Ida Chiara Guerrera

PROVIDER: PXD034222 | Pride | 2023-03-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	211224_SaCE_nE_n2-DIA_CL_CTRL_1_3039.d.zip	Other
	211224_SaCE_nE_n2-DIA_CL_CTRL_2_3040.d.zip	Other
	211224_SaCE_nE_n2-DIA_CL_CTRL_3_3041.d.zip	Other
	211224_SaCE_nE_n2-DIA_CL_CTRL_4_3042.d.zip	Other
	211224_SaCE_nE_n2-DIA_CL_CTRL_5_3043.d.zip	Other

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Publications

Promitotic Action of <i>Oenothera biennis</i> on Senescent Human Dermal Fibroblasts.

Ceccacci Sara S Roger Kévin K Metatla Ines I Chhuon Cerina C Tighanimine Khaled K Fumagalli Stefano S De Lucia Adriana A Pranke Iwona I Cordier Corinne C Monti Maria Chiara MC Guerrera Ida Chiara IC

International journal of molecular sciences 20221202 23

Accumulation of senescent dermal fibroblasts drives skin aging. The reactivation of proliferation is one strategy to modulate cell senescence. Recently, we reported the exact chemical composition of the hydrophilic extract of <i>Oenothera biennis</i> cell cultures (ObHEx) and we showed its skin anti-aging properties. The aim of this work is to assess its biological effect specifically on cell senescence. ObHEx action has been evaluated on normal human dermal fibroblasts subjected to stress-induc ...[more]

PMID: 36499490

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