Dataset Information

HeLa cells were transfected with MARVELD1, then treated with or without HU treatment,following with IP and LC-MSMS analysis

ABSTRACT: To analyze MARVELD1 interacting proteins, HeLa cells were transfected with MARVELD1-Flag or pcDNA3.1 (PC), then treated with 8 mM Hydroxyurea (MCE, USA) or DMSO for 24 h, and subjected to IP assays with anti-DYKDDDDK magnetic agarose (Thermo, USA). After washing five times with PBS buffer, samples were boiled in 2× SDS loading buffer, resolved in SDS-PAGE, visualized by Coomassie Blue staining, and subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis (PTM Bio, China, project number: FA203GI).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Haoxiu Sun

LAB HEAD: Haoxiu Sun

PROVIDER: PXD034445 | Pride | 2023-05-10

REPOSITORIES: Pride

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Publications

The regulation loop of MARVELD1 interacting with PARP1 in DNA damage response maintains genome stability and promotes therapy resistance of cancer cells.

Sun Haoxiu H Liu Chao C Han Fang F Lin Xiaoyu X Cao Liangyu L Liu Chenxing C Ji Qiuyu Q Cui Jinjin J Yao Yuanfei Y Wang Bojun B Liao Yuanyu Y Nie Huan H Zhang Yanqiao Y Li Yu Y

Cell death and differentiation 20230207 4

The DNA damage response (DDR) plays crucial roles in cancer prevention and therapy. Poly(ADP-ribose) polymerase 1 (PARP1) mediates multiple signal transduction in the DDR as a master regulator. Uncovering the regulatory factors of PARP1 contributes to a more comprehensive view of tumorigenesis and treatment strategies. Here, we reveal that MARVELD1 acts as a mediator of DDR to perform early events and maintain genome stability. Mechanistically, PARP1 PARylates MARVELD1 at D102, D118 and D130, an ...[more]

PMID: 36750717

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Dataset Information

HeLa cells were transfected with MARVELD1, then treated with or without HU treatment,following with IP and LC-MSMS analysis

Publications

The regulation loop of MARVELD1 interacting with PARP1 in DNA damage response maintains genome stability and promotes therapy resistance of cancer cells.

Similar Datasets

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