Proteomics

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Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA

ABSTRACT: Assessing the function of the RNA-binding activity of RNA-binding proteins (RBPs) is challenging. Here, Keil et al. use a targeted XL-ms approach to identify RNA-binding sites in components of nuclear mRNPs in S. cerevisiae. The analysis of RNA-binding mutants of one selected example, the SR-like protein Npl3, uncovers two novel functions of Npl3, namely in the recruitment of mRNP components to transcribed protein-coding genes and their transfer onto the mRNA. This data set applies label-free protein quantification to assess protein abundancies in NPL3 or CBC2 affinity purification experiments from cellular lysates of NPL3-WT and NPL3-P196D/A197D double-mutant expressing cells.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Henning Urlaub  

LAB HEAD: Prof. Dr. Henning Urlaub

PROVIDER: PXD034656 | Pride | 2023-03-11

REPOSITORIES: Pride

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Action DRS
20200827_Scerevisiae_UP_reviewed_canonical.fasta Fasta
MaxQuant_1.6.2.10.zip Other
Npl3_Link.fasta Fasta
P_Keil_060819_181019_Goe_Rep1_L7_1_1.raw Raw
P_Keil_060819_181019_Goe_Rep1_L7_1_10.raw Raw
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Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA.

Keil Philipp P   Wulf Alexander A   Kachariya Nitin N   Reuscher Samira S   Hühn Kristin K   Silbern Ivan I   Altmüller Janine J   Keller Mario M   Stehle Ralf R   Zarnack Kathi K   Sattler Michael M   Urlaub Henning H   Sträßer Katja K  

Nucleic acids research 20230101 2

RNA-binding proteins (RBPs) control every RNA metabolic process by multiple protein-RNA and protein-protein interactions. Their roles have largely been analyzed by crude mutations, which abrogate multiple functions at once and likely impact the structural integrity of the large ribonucleoprotein particles (RNPs) these proteins function in. Using UV-induced RNA-protein crosslinking of entire cells, protein complex purification and mass spectrometric analysis, we identified >100 in vivo RNA crossl  ...[more]

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