Dataset Information

Proteomic profiling skin mucus of European eel Anguilla anguilla infected with Anguillid herpesvirus

ABSTRACT: Anguillid herpesvirus 1 (AngHV) is an important viral pathogen of eel. This study was conducted to investigate the molecular mechanisms and immune response at the protein levels in the skin mucus of AngHV infected Anguilla anguilla. TMT-labelling proteomics with the liquid chromatographytandem mass spectrometry (LC-MS/MS) was used for protein quantitative identification. And the quantitative protein amount was detected by parallel reaction monitoring (PRM) analysis. A total of 3486 proteins were identified, of which 2935 were quantified. When a protein fold change greater than 1.3 or less than 0.76 was considered to indicate a differentially expressed protein (DEP), 187 up-regulated proteins and 126 down-regulated proteins were detected, and most of the DEPs were enriched in CAMs pathway, intestinal immune pathway, herpes simplex virus 1 infection pathway, phagosome pathway and p53 signaling pathway. The results of the DEPs detected by PRM were highly consistent with that of the TMT-labelled quantitative proteomic analysis results.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Anguilla Anguilla X Anguilla Rostrata

TISSUE(S): Skin Mucus

SUBMITTER: Ying-Ying Li

LAB HEAD: Jun-Qing Ge

PROVIDER: PXD034867 | Pride | 2023-03-10

REPOSITORIES: Pride

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Proteomic Profiling Skin Mucus of European Eel <i>Anguilla anguilla</i> Infected with Anguillid Herpesvirus.

Li Ying-Ying YY Yang Jin-Xian JX Chen Xi X Chen Qiang Q Song Tie-Ying TY Ge Jun-Qing JQ

International journal of molecular sciences 20220924 19

Anguillid herpesvirus 1 (AngHV) is an important viral pathogen affecting eel. This study was designed to investigate the potential molecular mechanisms and immune response elicited at the protein levels in the skin mucus of AngHV-infected <i>Anguilla anguilla</i>. Tandem mass tag (TMT)-labelling proteomics with the liquid chromatography tandem mass spectrometry (LC-MS/MS) was used for performing quantitative identification of the proteins. In addition, the quantitative protein amount was detecte ...[more]

PMID: 36232585

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