Proteomics

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Global proteomic profiling of multiple organs of cat (Felis catus) and proteome-transcriptome correlation during acute Toxoplasma gondii infection


ABSTRACT: Understanding altered expression of proteins and transcripts associated with Toxoplasma gondii infection in cats may improve our understanding of how this parasite manipulates the molecular microenvironment of the definitive host. We performed proteomics analysis of six organs (brain, heart, spleen, liver, lung and small intestine) in cats acutely infected with T. gondii. A total of 32,657 proteins were identified among the six examined organs, including 2,556 differentially expressed proteins (DEPs), of which 1,325 DEPs were up-regulated and 1,231 DEPs were down-regulated. The brain, liver, lung, spleen, heart and small intestine exhibited 125 DEPs, 463 DEPs, 255 DEPs, 283 DEPs, 855 DEPs and 675 DEPs, respectively. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were performed on all proteins and DEPs in all organs, showed that many proteins were enriched in binding, cell part, cell growth and death, signal transduction, translation, sorting and degradation and immune system. Correlation between proteins and transcripts with differential expression patterns were detected in the heart (n = 9), liver (n = 19), lung (n = 9), small intestine (n = 17), and spleen (n = 3). These DEPs were mainly involved in immune response, tryptophan catabolism, and extracellular matrix remodeling. Future investigations are needed to identify the pathophysiological mechanisms underlying the reported associations between the identified proteins and transcripts and T. gondii infection.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Felis Catus (cat) (felis Silvestris Catus)

TISSUE(S): Spleen, Heart, Brain, Liver, Lung, Epithelial Cell

DISEASE(S): Wounds And Injuries

SUBMITTER: Qinli Liang  

LAB HEAD: xing-quan zhu

PROVIDER: PXD033778 | Pride | 2022-10-15

REPOSITORIES: Pride

Dataset's files

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Identification.zip Other
P140561_1_1.mgf Mgf
P140561_1_1.wiff Wiff
P140561_1_1.wiff.scan Wiff
P140561_1_10.mgf Mgf
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Publications

Global proteomic profiling of multiple organs of cats (Felis catus) and proteome-transcriptome correlation during acute Toxoplasma gondii infection.

Nie Lan-Bi LB   Cong Wei W   He Jun-Jun JJ   Zheng Wen-Bin WB   Zhu Xing-Quan XQ  

Infectious diseases of poverty 20220914 1


<h4>Background</h4>Toxoplasma gondii is a protozoan parasite which can infect almost all warm-blooded animals and humans. Understanding the differential expression of proteins and transcripts associated with T. gondii infection in its definitive host (cat) may improve our knowledge of how the parasite manipulates the molecular microenvironment of its definitive host. The aim of this study was to explore the global proteomic alterations in the major organs of cats during acute T. gondii infection  ...[more]

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