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Deep and fast label-free Dynamic Organellar Mapping - DIA datasets

ABSTRACT: The Dynamic Organellar Maps (DOMs) approach combines cell fractionation and shotgun-proteomics for global profiling analysis of protein subcellular localization. Here, we have drastically enhanced the performance of DOMs through data-independent acquisition (DIA) mass spectrometry (MS). DIA-DOMs achieve twice the depth of our previous workflow in the same MS runtime, and substantially improve profiling precision and reproducibility. This repository contains all DIA-LFQ benchmark datasets that were measured with our optimized DIA methods: single shot or triple shot on 100 min, 44 min and 21 min gradients.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Julia Schessner

LAB HEAD: Georg H.H.

PROVIDER: PXD034971 | Pride | 2023-08-25

REPOSITORIES: Pride

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			Action	DRS
	1x100_discoveryDIA.zip	Other
	1x100_libDIA.zip	Other
	1x21_discoveryDIA.zip	Other
	1x44_discoveryDIA.zip	Other
	20201119_EXPL3_ViAl_SA_HeLa_DIA100min33w_MAP1_12K.raw	Raw

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