Proteomics

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A synthetic control genome provides a universal molecular standard to measure DNA, mRNA and protein expression


ABSTRACT: The phi X 174 bacteriophage was first sequenced in 1977, and has since become the most widely used standard in molecular biology and next-generation sequencing. However, with the advent of affordable DNA synthesis and de novo gene design, we considered whether we could engineer a synthetic genome, termed SynX, specifically tailored for use as a universal molecular standard. The SynX genome encodes 21 synthetic genes that can be in vitro transcribed to generate matched mRNA controls, and in vitro translated to generate matched protein controls. This enables the use of SynX as a matched control to compare across genomic, transcriptomic and proteomic experiments. The synthetic genes provide qualitative controls that measure sequencing accuracy across k-mers, GC-rich and repeat sequences, as well as act as quantitative controls that measure sensitivity and quantitative accuracy. We show how the SynX genome can measure DNA sequencing, evaluate gene expression in RNA sequencing experiments, or quantify proteins in mass spectrometry. Unlike previous spike-in controls, the SynX DNA, RNA and protein controls can be independently and sustainably prepared by recipient laboratories using common molecular biology techniques, and widely shared as a universal molecular standard.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Escherichia Coli

TISSUE(S): Cell Culture

SUBMITTER: Timothy McCubbin  

LAB HEAD: Tim Mercer

PROVIDER: PXD035035 | Pride | 2024-03-22

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20211117_SynX_peptide_experiment.sne Other
20211117_T2_008.raw Raw
20211117_T2_010.raw Raw
20211117_T2_012.raw Raw
20211210_T2_011.raw Raw
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