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Plasmodium falciparum PM X LC-MSMS

ABSTRACT: Plasmepsin X (PM X) is an aspartate protease expressed in the malaria parasie Plasmodium falciparum. Following synthesis PM X undergoes proteolytic processing. This project aims to understand the significance of PM X processing. To identify the cleavage sites, the processed PM X bands were excised from gels and subjected to LC-MSMS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Plasmodium Falciparum (isolate 3d7)

TISSUE(S): Cell Culture

SUBMITTER: Sumit Mukherjee

LAB HEAD: Daniel E. Goldberg

PROVIDER: PXD035172 | Pride | 2022-10-14

REPOSITORIES: Pride

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			Action	DRS
	20211116_Goldberg_2040_01_sumit1.raw	Raw
	20211116_Goldberg_2040_01_sumit1__F021288_.mzid.gz	Mzid
	20211116_Goldberg_2040_01_sumit1__F021288_.mzid_20211116_Goldberg_2040_01_sumit1__F021288_.MGF	Mzid
	20211116_Goldberg_2040_02_sumit2.raw	Raw
	20211116_Goldberg_2040_02_sumit2__F021289_.mzid.gz	Mzid

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Publications

Maturation and substrate processing topography of the Plasmodium falciparum invasion/egress protease plasmepsin X.

Mukherjee Sumit S Nguyen Suong S Sharma Eashan E Goldberg Daniel E DE

Nature communications 20220804 1

The malaria parasite Plasmodium invades a host erythrocyte, multiplies within a parasitophorous vacuole (PV) and then ruptures the PV and erythrocyte membranes in a process known as egress. Both egress and invasion are controlled by effector proteins discharged from specialized secretory organelles. The aspartic protease plasmepsin X (PM X) regulates activity for many of these effectors, but it is unclear how PM X accesses its diverse substrates that reside in different organelles. PM X also aut ...[more]

PMID: 35927261

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