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A genetically-encoded photo-proximity labeling approach for mapping protein territories

ABSTRACT: we introduce a genetically encoded system that allows protein interactomes to be captured using brief exposure to visible light. Our approach, Light-induced Interactome Tagging (LITag), involves fusing an engineered version of a plant flavoprotein (~12 kDa) to a protein of interest. Blue light excitation of the flavin mononucleotide (FMN) cofactor within the fusion protein leads to local generation of reactive radical species within exogenously added cell-permeable probes. When combined with quantitative proteomics, the system generates ‘snapshots’ of protein interactions with a temporal resolution of a few seconds.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Nir Hananya

LAB HEAD: Tom Muir

PROVIDER: PXD035265 | Pride | 2022-08-12

REPOSITORIES: Pride

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			Action	DRS
	COX4-peptides.txt	Txt
	COX4-proteinGroups.txt	Txt
	COX4_rev_4.raw	Raw
	Cox4_fwd_1.raw	Raw
	Cox4_fwd_3.raw	Raw

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