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Rapid Detection of Procalcitonin in septic serum by Immunoaffinity MALDI Chips

ABSTRACT: PCT concentration in the bloodstream correlates well with sepsis presence and in severe cases increases up to a thousand times from the healthy physiological values in a short time. In this study, we developed a rapid technique for PCT detection by MALDI-MS, that uses in-situ enrichment directly on the specialized immuno MALDI chips that are utilized directly as MALDI plates. The method’s ability to detect PCT was confirmed by comparing the results with LC-MS bottom-up workflow.

INSTRUMENT(S): maXis

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Petr Pompach

LAB HEAD: Petr Pompach

PROVIDER: PXD035456 | Pride | 2023-07-20

REPOSITORIES: Pride

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			Action	DRS
	Patient_1.csv	Csv
	Patient_1.mgf	Mgf
	Patient_10.csv	Csv
	Patient_10.mgf	Mgf
	Patient_11.csv	Csv

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Publications

The rapid detection of procalcitonin in septic serum using immunoaffinity MALDI chips.

Dvorak Josef J Novakova Jana J Kraftova Lucie L Studentova Vendula V Matejovic Martin M Radej Jaroslav J Karvunidis Thomas T Horak Jan J Kralovcova Marcela M Hrabak Jaroslav J Kalaninova Zuzana Z Volny Michael M Novak Petr P Pompach Petr P

Clinical proteomics 20230511 1

<h4>Background</h4>Sepsis is a common worldwide health condition with high mortality. It is caused by a dysregulated immune response to the pathogen. Severe infections resulting in sepsis can be also determined by monitoring several bloodstream biomarkers, one of them being pro-hormone procalcitonin (PCT). PCT concentration in the bloodstream correlates well with sepsis and in severe cases increases up to a thousand times from the healthy physiological values in a short time. In this study, we d ...[more]

PMID: 37170190

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