Proteomics

Dataset Information

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Loss of centrosomal gene ALMS1 alters lipid metabolism and the regulation of processes linked to the extracellular matrix.


ABSTRACT: In this project we evaluated the proteomic profiling with TGF-β stimuli at 24h in a CRISPR-Cas9 model for ALMS1 gene in hTERT-BJ-5ta cells. Proteomic results showed a majority inhibition of downstream regulated pathways by the TGF-β, associating the protein coding genes (PCG) with processes like TGF- β matrix regulation, epithelial mesenchymal transition (EMT), PI3K/AKT or P53. In conclusion, seems that the depletion of ALMS1 could be inhibiting the signals transduction through the TGF -β and the routes regulated downstream.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Erythrocyte, Cell Culture

DISEASE(S): Ciliopathy

SUBMITTER: Brais Bea Mascato  

LAB HEAD: Brais Bea-Mascato

PROVIDER: PXD035708 | Pride | 2024-01-26

REPOSITORIES: Pride

Dataset's files

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DPGDVP340140.pptx Other
DPGDVP340140_3525_proteins.xlsx Xlsx
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Publications

Loss of the centrosomal protein ALMS1 alters lipid metabolism and the regulation of extracellular matrix-related processes.

Bea-Mascato Brais B   Gómez-Castañeda Eduardo E   Sánchez-Corrales Yara E YE   Castellano Sergi S   Valverde Diana D  

Biology direct 20231208 1


<h4>Background</h4>Alström syndrome (ALMS) is a rare autosomal recessive disease that is associated with mutations in ALMS1 gene. The main clinical manifestations of ALMS are retinal dystrophy, obesity, type 2 diabetes mellitus, dilated cardiomyopathy and multi-organ fibrosis, characteristic in kidneys and liver. Depletion of the protein encoded by ALMS1 has been associated with the alteration of different processes regulated via the primary cilium, such as the NOTCH or TGF-β signalling pathways  ...[more]

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