Proteomics

Dataset Information

0

Mitochondrial interactome quantitation reveals structural changes in metabolic machinery in the failing murine heart


ABSTRACT: Advancements in cross-linking mass spectrometry (XL-MS) for structural analysis of proteins bridge the gap between purified systems and native tissue environments. Here, isobaric quantitative protein interaction reporter technology (iqPIR) was utilized to further extend XL-MS to the first system-wide comparative study of mitochondrial proteins from healthy and diseased murine hearts. The failing heart interactome includes 588 statistically significant cross-linked peptide pairs altered in the disease condition. Structural insight into ketone-oxidation metabolons, OXPHOS machinery, and nucleotide transporter hybrid-conformations support mitochondrial remodeling in failing hearts while bringing forth new hypotheses for pathological mechanisms. The application of quantitative cross-linking technology in tissue provides molecular-level insight into complex biological systems challenging to model in cell culture, thus providing a valuable resource for studying human diseases.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Anna Bakhtina  

LAB HEAD: James Edward Bruce

PROVIDER: PXD035712 | Pride | 2023-02-27

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
040522_0316_7_sham_2h_1.raw Raw
040522_0316_7_sham_2h_2.raw Raw
040522_0317_11_Tac_2h_1.raw Raw
040522_0317_11_Tac_2h_2.raw Raw
040522_0357_1_sham_2h_1.raw Raw
Items per page:
1 - 5 of 27

Similar Datasets

2022-08-02 | PXD035622 | Pride
2022-08-02 | PXD027757 | Pride
2022-03-17 | PXD027234 | Pride
2021-09-24 | PXD027611 | Pride
2022-12-03 | PXD036600 |
2015-01-01 | GSE57338 | GEO
2015-01-01 | GSE57344 | GEO
2024-10-17 | PXD043531 | Pride
2016-02-08 | E-GEOD-76701 | biostudies-arrayexpress
2023-06-26 | PXD043282 |