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A deep XL-proteomic dataset of intact HEK293T cells using DSBSO

ABSTRACT: Cross-linking mass spectrometry is an emerging method to study protein-protein interactions (PPIs) in intact biological systems. Here, we optimized a workflow for gnerating high coverage maps of PPIs using the enrichable cross-linker Azide-A-DSBSO. This created one of the largest to-date XL-MS datasets, enabling structural characterization of thousands of PPIs in their intact subcellular environment.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney

SUBMITTER: Boris Bogdanow

LAB HEAD: Fan Liu

PROVIDER: PXD043531 | Pride | 2024-10-17

REPOSITORIES: Pride

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Fast and highly efficient affinity enrichment of Azide-A-DSBSO cross-linked peptides.

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