Proteomics

Dataset Information

0

Crosstalk of Protein Expression and Lysine Acetylation in Response to TMV Infection in Nicotiana benthamiana


ABSTRACT: Kac, a reversible PTM, plays essential roles in various biological processes, including those involving metabolic pathways, pathogen resistance and transcription, in both prokaryotes and eukaryotes. TMV, the major factor that causes poor quality of Solanaceae crops worldwide, directly alters many metabolic processes in tobacco. However, the extent and function of Kac during TMV infection have not been determined. Here, using LC−MS/MS in conjunction with highly sensitive immune-affinity purification, we comprehensively analyzed the changes in the proteome and acetylome of TMV-infected tobacco (Nicotiana benthamiana) seedlings. In total, 2082 lysine-acetylated sites on 1319 proteins differentially expressed in response to TMV infection were identified. Extensive bioinformatic studies disclosed changes in acetylation of proteins engaged in cellular metabolism and biological processes. The vital influence of Kac in fatty acid degradation and alpha-linolenic acid metabolism was also revealed in TMV-infected seedlings. This study first revealed Kac information in N. benthamiana under TMV infection and expands upon the existing landscape of acetylation in pathogen infection.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Nicotiana Tabacum (common Tobacco)

TISSUE(S): Leaf

SUBMITTER: Xianliang Ma  

LAB HEAD: Jinguang Yang

PROVIDER: PXD035737 | Pride | 2022-10-15

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
QE_HFX_2020928_50cm_60min_Fr1.raw Raw
QE_HFX_2020928_50cm_60min_Fr10.raw Raw
QE_HFX_2020928_50cm_60min_Fr11.raw Raw
QE_HFX_2020928_50cm_60min_Fr12.raw Raw
QE_HFX_2020928_50cm_60min_Fr13.raw Raw
Items per page:
1 - 5 of 16
altmetric image

Publications

Cross-Talk of Protein Expression and Lysine Acetylation in Response to TMV Infection in <i>Nicotiana benthamiana</i>.

Song Liyun L   Zhan Huaixu H   Wang Yujie Y   Lin Zhonglong Z   Li Bin B   Shen Lili L   Jiao Yubing Y   Li Ying Y   Wang Fenglong F   Yang Jinguang J  

ACS omega 20220901 36


Lysine acetylation (K<sup>ac</sup>), a reversible PTM, plays an essential role in various biological processes, including those involving metabolic pathways, pathogen resistance, and transcription, in both prokaryotes and eukaryotes. TMV, the major factor that causes the poor quality of Solanaceae crops worldwide, directly alters many metabolic processes in tobacco. However, the extent and function of K<sup>ac</sup> during TMV infection have not been determined. The validation test to detect K<s  ...[more]

Similar Datasets

2024-08-16 | GSE272661 | GEO
2014-05-12 | E-GEOD-47180 | biostudies-arrayexpress
2017-07-17 | GSE97663 | GEO
2014-11-03 | E-MEXP-3934 | biostudies-arrayexpress
2012-02-02 | E-GEOD-34841 | biostudies-arrayexpress
2018-11-21 | PXD011633 | Pride
2018-11-07 | PXD008837 | Pride
2021-05-12 | PXD022517 | Pride
2009-03-24 | E-GEOD-12918 | biostudies-arrayexpress
2014-05-12 | GSE47180 | GEO