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Chemical crosslinking of the SPATA5-SPATA5L1-C1orf109-CINP complex

ABSTRACT: Here we present data using chemical crosslinking to inform on the architecture of the SPATA5-SPATA5L1-C1orf109-CINP complex (or the 55LCC).

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Antonio Calabrese

LAB HEAD: Antonio Calabrese

PROVIDER: PXD035980 | Pride | 2024-04-17

REPOSITORIES: Pride

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			Action	DRS
	fbsaca_220705_xl_50cm_02.raw	Raw
	fbsaca_220705_xl_50cm_03.raw	Raw
	new-combined.zhrm	Other

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Publications

The SPATA5-SPATA5L1 ATPase complex directs replisome proteostasis to ensure genome integrity.

Krishnamoorthy Vidhya V Foglizzo Martina M Dilley Robert L RL Wu Angela A Datta Arindam A Dutta Parul P Campbell Lisa J LJ Degtjarik Oksana O Musgrove Laura J LJ Calabrese Antonio N AN Zeqiraj Elton E Greenberg Roger A RA

Cell 20240329 9

Ubiquitin-dependent unfolding of the CMG helicase by VCP/p97 is required to terminate DNA replication. Other replisome components are not processed in the same fashion, suggesting that additional mechanisms underlie replication protein turnover. Here, we identify replisome factor interactions with a protein complex composed of AAA+ ATPases SPATA5-SPATA5L1 together with heterodimeric partners C1orf109-CINP (55LCC). An integrative structural biology approach revealed a molecular architecture of SP ...[more]

PMID: 38554706

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Mechanism of 60S ribosomal subunit recognition and UFMylation by the UFM1 E3 ligase complex

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2024-03-22 | PXD046990 | Pride

Surgical trauma in mice modifies the content of circulating extracellular vesicles

Project description:Surgical interventions rapidly trigger a cascade of molecular, cellular and neural signaling responses that ultimately reach remote organs, including the brain. Using a mouse model of orthopedic surgery, we have previously demonstrated hippocampal metabolic, structural, and functional changes associated with cognitive impairment. However, the nature of the underlying signals responsible for such periphery-to-brain communication remains hitherto elusive. This is the first exploratory study that tests the hypothesis of extracellular vesicles (EVs) as potential mediators carrying information from the injured tissue to the distal organs including the brain. The primary goal was to investigate whether the cargo of circulating EVs after surgery can undergo quantitative changes that could potentially trigger phenotypic modifications in the target tissues. EVs were isolated from the serum of the mice subjected to a tibia surgery after 6, 24, and 72 h, and the proteome and miRNAome were investigated using mass spectrometry and RNA-seq approaches. We found substantial differential expression of proteins and miRNAs starting at 6 h post-surgery and peaking at 24 h. Interestingly, one of the up-regulated proteins at 24 h was α-synuclein, a pathogenic hallmark of certain neurodegenerative syndromes. We conclude that surgery alters the cargo of circulating EVs in the blood, and our results suggest EVs as potential systemic signal carriers mediating remote effects of surgery on the brain.

2022-01-08 | PXD030167 | Pride

Characterization of the NTSR1-Arr2 complex

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COVIDomics_plasma_2020_DDA-PASEF

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2021-03-04 | PXD022817 | Pride

Hypoxia reveals the new function of Foxn1 in keratinocytes antioxidant defense system

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2023-03-08 | PXD039474 | Pride

Mass spectrometry-based proteomic exploration of diverse murine macrophage cellular models

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