Recognition of DNA damage lesions across the tree of life

ABSTRACT: Protecting the integrity of their genome is critical to all species. The surveillance and maintenance of the genome of every organism is orchestrated by a highly regulated combination of DNA damage response and specific DNA repair pathways. Two of these critical pathways are base excision repair (BER) and ribonucleotide excision repair (RER). Here, we investigate the phylogenetic diversity in the recognition and repair of three well-established DNA lesions, primarily repaired by BER or RER: 1) 8-oxoguanine, 2) an abasic site, and 3) incorporated ribonucleotide in DNA in E. coli, B. subtilis, H. salinarum, T. brucei, T. thermophila, S. cerevisiae, S. pombe, C. elegans, human HeLa and HEK293 cells, A. thaliana, and Z. mays. Using quantitative mass spectrometry, we identified 337 enriched interactors across these species. Of these proteins, 140 were previously characterized to be involved in DNA repair, and 39 proteins were newly identified due to their homology to previously studied repair proteins. For 10 of the 11 species, we identify unique, uncharacterized proteins that lack homology to any protein within the included 11 species. Our study emphasizes strong crosstalk between RER and BER and highlights the evolutionary conservation of this cross recognition across all domains of life.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Caenorhabditis Elegans Trypanosoma Brucei Saccharomyces Cerevisiae (baker's Yeast) Arabidopsis Thaliana (mouse-ear Cress) Zea Mays (maize) Halobacterium Salinarum

SUBMITTER: F Butter  

LAB HEAD: Falk Butter

PROVIDER: PXD036040 | Pride | 2023-07-20

REPOSITORIES: Pride