Proteomics

Dataset Information

0

Identification of phosphorylation sites of HA-ORP8 immunoprecipitated from cells


ABSTRACT: To candidate phosphorylation sites of ORP8 in cells and the stoichiometric change of ORP8's phosphorylation when AMPK is activated,LC-MS/MS analysis of ORP8 immunoprecipitated from serum-starved cells and cells treated with AMPK activator AICAR was performed.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: maomao pu  

LAB HEAD: Wei Liu

PROVIDER: PXD036204 | Pride | 2023-10-24

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
liuwei149-peptides_1_1_0.mzid.gz Mzid
liuwei149.mgf Mgf
liuwei149.raw Raw
liuwei150-peptides_1_1_0.mzid.gz Mzid
liuwei150.mgf Mgf
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Publications

ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover.

Pu Maomao M   Zheng Wenhui W   Zhang Hongtao H   Wan Wei W   Peng Chao C   Chen Xuebo X   Liu Xinchang X   Xu Zizhen Z   Zhou Tianhua T   Sun Qiming Q   Neculai Dante D   Liu Wei W  

Protein & cell 20230901 9


Lipophagy, the selective engulfment of lipid droplets (LDs) by autophagosomes for lysosomal degradation, is critical to lipid and energy homeostasis. Here we show that the lipid transfer protein ORP8 is located on LDs and mediates the encapsulation of LDs by autophagosomal membranes. This function of ORP8 is independent of its lipid transporter activity and is achieved through direct interaction with phagophore-anchored LC3/GABARAPs. Upon lipophagy induction, ORP8 has increased localization on L  ...[more]

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