Project description:To determine the effect of CRISPR interference-mediated silencing of glycosylation in Burkholderia thailandensis harbouring a pglL-targeted guide, and a nontarget guide using Data Independent Acquisition
Project description:To determine whether CRISPR interference can be used to silence the expression of pglL in Burkholderia diffusa and therefore modulate glycosylation
Project description:To determine the effect of CRISPR interference-mediated silencing of glycosylation in Burkholderia multivorans harbouring a pglL-targeted guide, and a nontarget guide using Data Independent Acquisition
Project description:To determine the affect of CRISPR interference-mediated silencing of glycosylation in Burkholderia diffusa harbouring a pglL (WL85_RS02125)-targeted guide, and a nontarget guide using Data Independent Acquisition
Project description:To determine whether CRISPR interference can be used to recapitulate a glycosylation null mutant strain in Burkholderia cenocepacia via data independent acquisition mass spectrometry using a low level of dCas9 induction. A guideless plasmid was containing in the wildtype and pglL mutant strains, and the strains harbouring the dCas9 insertion containing a pglL-targeting plasmid Bc-pglL-C, non-target, and the guideless plasmid.
Project description:To interrogate the glycoproteome following knockdown of pglL and subsequent knockdown of glycosylation in Burkholderia thailandensis through Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) glycopeptide enrichment – loading control pre-enrichment
Project description:To determine a suitable level of rhamnose required for the effective silencing of pglL and glycosylation using CRISPR interference in Burkholderia cenocepacia
Project description:To determine whether CRISPR interference can be used to recapitulate a glycosylation null mutant strain in Burkholderia cenocepacia via data independent acquisition mass spectrometry