Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Validating the use of CRISPR interference for the modulation of glycosylation in Burkholderia cenocepacia - induction levels

ABSTRACT: To determine a suitable level of rhamnose required for the effective silencing of pglL and glycosylation using CRISPR interference in Burkholderia cenocepacia

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Burkholderia Cenocepacia K56-2valvano

SUBMITTER: Jessica Lewis

LAB HEAD: Nichollas Scott

PROVIDER: PXD036568 | Pride | 2023-03-20

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	CRISPRi_induction_Bcenocepacia.zip	Other
	Nsco_FAIMS_glycopeptide_CRISPRi_induction_exp_B1.raw	Raw
	Nsco_FAIMS_glycopeptide_CRISPRi_induction_exp_B10.raw	Raw
	Nsco_FAIMS_glycopeptide_CRISPRi_induction_exp_B11.raw	Raw
	Nsco_FAIMS_glycopeptide_CRISPRi_induction_exp_B12.raw	Raw

Items per page:

1 - 5 of 30

Similar Datasets

Direct comparison between a Burkholderia cenocepacia glycosylation mutant strain, and a glycosylation knockdown strain

2023-03-20 | PXD037550 | Pride

Validating the use of CRISPR interference for the modulation of glycosylation in Burkholderia diffusa

Project description:To determine whether CRISPR interference can be used to silence the expression of pglL in Burkholderia diffusa and therefore modulate glycosylation

2023-03-20 | PXD036585 | Pride

Validating the use of CRISPR interference for the modulation of glycosylation in Burkholderia cenocepacia

Project description:To determine whether CRISPR interference can be used to modulation glycosylation in Burkholderia cenocepacia

2023-03-20 | PXD036564 | Pride

Direct comparison between a Burkholderia cenocepacia glycosylation mutant, and a CRISPRi-mediated glycosylation knockdown strain

Project description:To determine whether CRISPR interference can be used to recapitulate a glycosylation null mutant strain in Burkholderia cenocepacia via data independent acquisition mass spectrometry using a low level of dCas9 induction. A guideless plasmid was containing in the wildtype and pglL mutant strains, and the strains harbouring the dCas9 insertion containing a pglL-targeting plasmid Bc-pglL-C, non-target, and the guideless plasmid.

2023-03-20 | PXD038043 | Pride

ii. Analysing the effects of glycosylation knockdown via CRISPR interference in Burkholderia multivorans

Project description:To determine the effect of CRISPR interference-mediated silencing of glycosylation in Burkholderia multivorans harbouring a pglL-targeted guide, and a nontarget guide using Data Independent Acquisition

2023-03-20 | PXD037585 | Pride

Identifying the glycoproteome of Burkholderia cenocepacia following knockdown of glycosylation using CRISPR interference (low dCas9 induction) - loading control for glycopeptide enrichment

Project description:To interrogate the glycoproteome following knockdown of pglL and subsequent knockdown of glycosylation in Burkholderia cenocepacia through Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) glycopeptide enrichment with 0.1% rhamnose for dCas9 induction– loading control pre-enrichment. Using strains harbouring the pglL-targeting guide C (Bc-pglL-C) and guideless plasmids.

2023-03-20 | PXD038044 | Pride

Validating the use of CRISPR interference for the modulation of glycosylation in Burkholderia thailandensis

Project description:To determine whether CRISPR interference can be used to modulate glycosylation in Burkholderia thailandensis

2023-03-20 | PXD036623 | Pride

Validating the use of CRISPR interference for the modulation of glycosylation in Burkholderia multivorans

Project description:To determine whether CRISPR interference can be used to modulation glycosylation in Burkholderia multivorans

2023-03-20 | PXD036619 | Pride

Identifying the glycoproteome of Burkholderia cenocepacia following knockdown of glycosylation using CRISPR interference (low dCas9 induction)

Project description:To interrogate the glycoproteome following knockdown of pglL and subsequent knockdown of glycosylation in Burkholderia cenocepacia through Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) glycopeptide enrichment with 0.1% rhamnose for dCas9 induction. Using strains harbouring the pglL-targeting guide C (Bc-pglL-C) and guideless plasmids.

2023-03-20 | PXD040181 | Pride

Identifying the glycoproteome of Burkholderia cenocepacia following knockdown of glycosylation using CRISPR interference (low dCas9 induction)

Project description:To interrogate the glycoproteome following knockdown of pglL and subsequent knockdown of glycosylation in Burkholderia cenocepacia through Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) glycopeptide enrichment with 0.1% rhamnose for dCas9 induction. Using strains harbouring the pglL-targeting guide C (Bc-pglL-C) and guideless plasmids.

2023-03-20 | PXD038047 | Pride