Dataset Information

Phosphorylation of purified recombinant mouse Ric-8B

ABSTRACT: Analysis of recombinant mouse Ric-8B protein phosphorylation. Ric-8B was purified from insect cells and E.coli and both treated with protein kinase CK2 and re-purified prior to LC-MS/MS. Another Ric-8B sample from insect cells was not treated with CK2 and also subjected to LC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Frank Kwarcinski

LAB HEAD: Gregory G. Tall

PROVIDER: PXD036645 | Pride | 2024-05-23

REPOSITORIES: Pride

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			Action	DRS
	MSB52141Chym041921.raw	Raw
	MSB52141Chym041921__F174092_.mzid.gz	Mzid
	MSB52141Chym041921__F174092_.mzid_MSB52141Chym041921__F174092_.MGF	Mzid
	MSB52141Tryp041921.raw	Raw
	MSB52141Tryp041921__F174089_.mzid.gz	Mzid

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Publications

Structures of Ric-8B in complex with Gα protein folding clients reveal isoform specificity mechanisms.

Papasergi-Scott Makaía M MM Kwarcinski Frank E FE Yu Maiya M Panova Ouliana O Ovrutsky Ann M AM Skiniotis Georgios G Tall Gregory G GG

Structure (London, England : 1993) 20230316 5

Mammalian Ric-8 proteins act as chaperones to regulate the cellular abundance of heterotrimeric G protein α subunits. The Ric-8A isoform chaperones Gαi/o, Gα12/13, and Gαq/11 subunits, while Ric-8B acts on Gαs/olf subunits. Here, we determined cryoelectron microscopy (cryo-EM) structures of Ric-8B in complex with Gαs and Gαolf, revealing isoform differences in the relative positioning and contacts between the C-terminal α5 helix of Gα within the concave pocket formed by Ric-8 α-helical repeat el ...[more]

PMID: 36931277

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