Dataset Information

Proteomic study of a breast cancer cell line following hemin treatment

ABSTRACT: Heme, an iron-containing prosthetic group found in many proteins, carries out diverse biological functions such as electron transfer, oxygen storage and enzymatic reactions. Hemin, the oxidised form of heme, is used to treat porphyria and also to activate heme-oxygenase (HO) which catalyses the rate-limiting step in heme degradation. Our group has previously demonstrated that hemin displays antitumor activity in breast cancer (BC). The aim of this work has been to study the effect of hemin on protein expression modifications in a BC cell line to gain insight into the molecular mechanisms of hemin antitumor activity. For this purpose, we carried out proteome analysis by Mass Spectrometry (MS) which showed that 1309 proteins were significantly increased in hemin-treated cells, including HO-1 and the proteases that regulate HO-1 function, and 921 proteins were significantly decreased. Furthermore, the MS-data analysis showed that hemin regulates the expression of heme- and iron- related proteins, adhesion and cytoskeletal proteins, cancer signal transduction proteins and enzymes involved in lipid metabolism. By biochemical and cellular studies, we further corroborated the most relevant in-silico results. Altogether, these results show the multiple physiological effects that hemin treatment displays in BC and demonstrate its potential as anticancer agent.

INSTRUMENT(S): Thermo Scientific instrument model

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: GEORGINA COLO

LAB HEAD: Alejandro Curino

PROVIDER: PXD036701 | Pride | 2023-07-20

REPOSITORIES: Pride

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			Action	DRS
	20190513_coreQEP2_AnWe_LC12-17_SA_Orbi3208Sample01.raw	Raw
	20190513_coreQEP2_AnWe_LC12-17_SA_Orbi3208Sample02.raw	Raw
	20190513_coreQEP2_AnWe_LC12-17_SA_Orbi3208Sample03.raw	Raw
	20190513_coreQEP2_AnWe_LC12-17_SA_Orbi3208Sample04.raw	Raw
	20190513_coreQEP2_AnWe_LC12-17_SA_Orbi3208Sample05.raw	Raw

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Proteomic analysis of the effect of hemin in breast cancer.

Coló G P GP Schweitzer K K Oresti G M GM Alonso E G EG Chávez L Fernández LF Mascaró M M Giorgi G G Curino A C AC Facchinetti M M MM

Scientific reports 20230621 1

Heme, an iron-containing prosthetic group found in many proteins, carries out diverse biological functions such as electron transfer, oxygen storage and enzymatic reactions. Hemin, the oxidised form of heme, is used to treat porphyria and also to activate heme-oxygenase (HO) which catalyses the rate-limiting step in heme degradation. Our group has previously demonstrated that hemin displays antitumor activity in breast cancer (BC). The aim of this work has been to study the effect of hemin on pr ...[more]

PMID: 37344532

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