Proteomics

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Production and properties of adhesin-free gingipain proteinase RgpA


ABSTRACT: To obtain RgpA free of adhesin requires a means of separating the RgpA catalytic domain from the adhesins domains or adaptation of a method to prevent RgpA complex association.In this study, recombinant suicide plasmids were designed for use in homologous recombination with the rgpA locus so as to produce modified RgpA that would release from the P. gingivalis surface, thus enabling RgpA purification from the culture fluid.

INSTRUMENT(S): Q-Tof Ultima

ORGANISM(S): Porphyromonas Gingivalis W50

SUBMITTER: Abu Sayeed Mohammad Mahmud  

LAB HEAD: Eric C Reynolds

PROVIDER: PXD036859 | Pride | 2023-06-02

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
180501_Sayeed_10.raw Raw
180501_Sayeed_11.raw Raw
180501_Sayeed_12.raw Raw
180501_Sayeed_13.raw Raw
180501_Sayeed_14.raw Raw
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