Dataset Information

Lateral interactions between B7-H3 and CD147 are essential for stemness in breast cancer: a novel insight from proximal proteome analysis

ABSTRACT: Oncogenic cell-surface membrane proteins contribute to the phenotypic and functional characteristics of cancer stem cells (CSCs). We quantitatively analyzed cell-surface membrane proteins that are in close proximity to CD147 in CSCs using proximity-labeling proteomic approach. Moreover, we compared CSCs to non-CSCs to identify CSC-specific cell-surface membrane proteins that are the nearest neighbors of CD147 and revealed that lateral interaction between CD147 and CD276 (B7H3) concealed within the lipid raft microdomain in CSCs confers resistance to docetaxel, known to treat many types of cancer patients including metastatic breast cancer. Furthermore, we found that co-expression of CD147 and CD276 in patients with HER2+ breast cancer who received chemotherapy had poor disease-free survival (DFS) and Overall survival (OS) rates (p = 0.04 and 0.08, respectively). Subsequent immunohistochemical analysis indicated that co-expression of CD147 and CD276 may be associated with poor response to chemotherapy in an independent HER2+ BC cohort. Altogether, our study suggests that the lateral interaction between CD147 and its proximal partners, such as CD276, may be related to a poor prognostic factor in BC and a predictive marker for the critical phenotypic determinant of breast cancer stemness.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Eugene C Yi

LAB HEAD: Eugene C. Yi

PROVIDER: PXD036909 | Pride | 2023-10-24

REPOSITORIES: Pride

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			Action	DRS
	20180205_set1_SPPLAT_2B9_NCSC_F1.raw	Raw
	20180205_set1_SPPLAT_2B9_NCSC_F2.raw	Raw
	20180205_set1_SPPLAT_2B9_NCSC_F3.raw	Raw
	20180205_set1_SPPLAT_2B9_NCSC_F4.raw	Raw
	20180205_set1_SPPLAT_2B9_NCSC_F5.raw	Raw

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Lateral interactions between CD276 and CD147 are essential for stemness in breast cancer: a novel insight from proximal proteome analysis.

Seo Yu Ri YR Lee Junghyeon J Ryu Han Suk HS Kim EunHee G EG Kim So Hyun SH Jeong Jieun J Jung Hyeryeon H Jung YeoJin Y Kim Han Byeol HB Jo Yeon Hui YH Kim Yeong Dong YD Jin Min-Sun MS Lee Yong Yook YY Kim Kristine M KM Yi Eugene C EC

Scientific reports 20230830 1

Oncogenic cell-surface membrane proteins contribute to the phenotypic and functional characteristics of cancer stem cells (CSCs). We employed a proximity-labeling proteomic approach to quantitatively analyze the cell-surface membrane proteins in close proximity to CD147 in CSCs. Furthermore, we compared CSCs to non-CSCs to identify CSC-specific cell-surface membrane proteins that are closely interact with CD147 and revealed that lateral interaction between CD147 and CD276 concealed within the li ...[more]

PMID: 37648771

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