Tear proteome revealed association of S100A family proteins and mesothelin with thrombosis in elderly patients with retinal vein occlusion

ABSTRACT: Tear samples collected from patients with central retinal vein occlusion (n=58) and group of healthy volunteers (n=30) were analyzed using a proteomic label-free absolute quantitative ap-proach. We found a large proportion (458 proteins with a frequency > 0.6) of tear proteome shared between healthy donors and subjects with the central retinal vein occlusion (CRVO). Comparative proteomic analysis revealed 29 proteins (p<0.05) significantly differed in their quantitative property between CRVO patients and the control group. Among them S100A6 (log (2)FC = 1.11, p<0.001), S100A8 (log(2)FC = 2.45, p<0.001), and S100A9 (log2(FC) = 2.08, p<0.001) are the most abundantly represented upregulated proteins, and β2-microglobulin was the most downregulated protein (log2(FC) = -2.13, p<0.001). Mesothelin (MSLN) was found as a protein with a significant increase (log2(FC) = 0.82, p<0.001) in the CRVO group. The selected up- and downregulated pro-teins were assembled to customize map of the CRVO-related critical protein interactions with quantitative properties. The customized map (FDR<0.01) emerged inflammation, impairment of retinal hemostasis, and immune response as the main set of processes associated with CRVO is-chemic condition. The semantic analysis encouraged the prevalence of core biological processes encompassing dysregulation of mitochondrial organization and regulation of improperly or utilization of topologically incorrect folded proteins as a consequence of oxidative stress and in-flammation escalating the ischemic condition caused by the local retinal hemostasis dysregulation. The most significantly different proteins (S100A6, S100A8, S100A9, MSLN, and β2-microglobulin) were applied for the ROC plotting and their AUC varied from 0.772 to 0.952 suggesting association with the CRVO.

INSTRUMENT(S): Xevo G2-XS Tof

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Tear

DISEASE(S): Central Retinal Vein Occlusion

SUBMITTER: Arthur Kopylov  

LAB HEAD: Anna L. Kaysheva

PROVIDER: PXD037021 | Pride | 2022-12-13

REPOSITORIES: Pride