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The proteomic content of Varroa destructor gut varies according to the developmental stage of its host

ABSTRACT: Varroa destructor is one of the major parasitic pests in modern beekeeping worldwide. Since it shifted host from Asian to Western honey bees, it was shown to weaken colonies by feeding on both immature and adult stages while transmitting several deadly viruses in the process. Nutrition, an overlooked aspect of parasite biology, is thus a key to comprehend the V. destructor life cycle and its impacts on its honey bee host. We explored the feeding physiology of this ectoparasite both through artificial feeding and by dissecting their gut and analysing their protein content especially in terms of host-derived nutrients. We found that the mite requires higher quantities of carbohydrates than proteins and that the nature of these proteins is crucial for its survival. The abundance of these essential nutrients such as Vitellogenin or Hexamerin varies throughout the bee development and could directly impact the parasite physiology. The analysis and identification of key proteins required for the miteâ€™s survival and reproduction will pave the way for the development of more specific control strategies.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Varroa Destructor Apis Mellifera (honeybee)

TISSUE(S): Hemolymph, Fat Body

SUBMITTER: Karim Arafah

LAB HEAD: Karim ARAFAH

PROVIDER: PXD053798 | Pride | 2024-12-16

REPOSITORIES: Pride

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			Action	DRS
	EKAR_NSI_F631.mzML	Mzml
	EKAR_NSI_F631.mzid.gz	Mzid
	EKAR_NSI_F631.raw	Raw
	EKAR_NSI_F634.mzML	Mzml
	EKAR_NSI_F634.mzid.gz	Mzid

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