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Identification of mono-ADPr readers by multilevel chromatin proteomics

ABSTRACT: We performed multilevel proteomics to identify mono-ADPr readers. Pulldowns using ADPribosylated peptides and nucleosomes, analysis of the chromatin proteome using targeted modulations of the ADPr system (H2O2 treatment, ARH3 KO, HPF1 KO). We reveal multiple mono-ADPr readers, including RNF114, a ubiquitin ligase recruited to DNA lesions through a zinc finger domain.

INSTRUMENT(S): Orbitrap Fusion Lumos, Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Thomas Colby

LAB HEAD: Ivan Matic

PROVIDER: PXD037026 | Pride | 2023-04-28

REPOSITORIES: Pride

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			Action	DRS
	170928_QE_JJB_PD_H3S28ad_Light_Mod.raw	Raw
	170928_QE_JJB_PD_H3S28ad_Light_Unm.raw	Raw
	171005_QE_JJB_PD_H3S10ad_Light_Mod.raw	Raw
	171005_QE_JJB_PD_H3S10ad_Light_Unm.raw	Raw
	171208_QE_JJB_PD_H4S1ad_Light_Mod.raw	Raw

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