Identification of mono-ADPr readers by multilevel chromatin proteomics
Ontology highlight
ABSTRACT: We performed multilevel proteomics to identify mono-ADPr readers. Pulldowns using ADPribosylated peptides and nucleosomes, analysis of the chromatin proteome using targeted modulations of the ADPr system (H2O2 treatment, ARH3 KO, HPF1 KO). We reveal multiple mono-ADPr readers, including RNF114, a ubiquitin ligase recruited to DNA lesions through a zinc finger domain.
INSTRUMENT(S): Orbitrap Fusion Lumos, Orbitrap Fusion
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: Thomas Colby
LAB HEAD: Ivan Matic
PROVIDER: PXD037026 | Pride | 2023-04-28
REPOSITORIES: Pride
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