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: Proteomic analysis of Nicotiana benthamiana upon infection of grapevine fanleaf virus strains

ABSTRACT: Nicotiana benthamiana was infected with several strains of grapevine fanleaf virus (GFLV). Apical tissue was collected 4, 7, and 12 days after inoculation, with identical samples for shotgun proteomics and transcriptomics analysis. Five leaf discs per leaf were collected a pooled by three plants into a single tube at each time point. Five biological replicates represent each treatment at each time point for a total of 75 samples. Two samples were lost between sample processing and data acquisition. The analysis methods between proteomics and transcriptomics were then cross-analyzed for host genes responsible for phenotypic differences upon infection.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Nicotiana Benthamiana

TISSUE(S): Leaf

SUBMITTER: Brandon Roy

LAB HEAD: Michelle Heck

PROVIDER: PXD037185 | Pride | 2023-07-20

REPOSITORIES: Pride

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			Action	DRS
	10a.dat	Other
	10b.dat	Other
	11a.dat	Other
	11b.dat	Other
	12a.dat	Other

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Publications

Profiling Plant Proteome and Transcriptome Changes during Grapevine Fanleaf Virus Infection.

Roy Brandon G BG DeBlasio Stacy S Yang Yong Y Thannhauser Theodore T Heck Michelle M Fuchs Marc M

Journal of proteome research 20230426 6

Viruses can elicit varying types and severities of symptoms during plant host infection. We investigated changes in the proteome and transcriptome of <i>Nicotiana benthamiana</i> plants infected by grapevine fanleaf virus (GFLV) with an emphasis on vein clearing symptom development. Comparative, time-course liquid chromatography tandem mass spectrometry and 3' ribonucleic acid sequencing analyses of plants infected by two wildtype GFLV strains, one symptomatic and one asymptomatic, and their asy ...[more]

PMID: 37099450

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