Proteomics

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Peptidomic analysis of endogenous and bacterial protease activity in human plasma

ABSTRACT: The clinical assessment of wound infection and related complications is challenging and the development of objective methods to measure wound status and predict healing outcomes is therefore essential. As endogenous and bacterial protease activities play important roles in healing and infection, analysis of changes in the wound peptidome by individual enzymes could provide insight into proteolytic events occurring in wounds, and may aid in the discovery of biomarkers. We mimicked wound fluid formation and wound infection by digesting plasma ex vivo with endogenous (human neutrophil elastase and Cathepsin G) and bacterial proteases (Pseudomonas aeruginosa LasB and Staphyloccocus aureus V8). Using a peptidomics approach, we characterized the low-molecular-weight peptidome of these samples and identified over 100 protein targets for each enzyme, and found enzyme-specific peptides and digestion patterns.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

DISEASE(S): Wounds And Injuries

SUBMITTER: Mariena van der Plas  

LAB HEAD: Mariena J.A.

PROVIDER: PXD037245 | Pride | 2024-03-07

REPOSITORIES: Pride

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Dataset's files

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Action DRS
20210903_EV_SS_Jun_CP1_0.raw Raw
20210903_EV_SS_Jun_CP1_24.raw Raw
20210903_EV_SS_Jun_CP1_H_V8.raw Raw
20210903_EV_SS_Jun_CP1_H_lasB.raw Raw
20210903_EV_SS_Jun_CP1_L_V8.raw Raw
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Peptidomic analysis of endogenous and bacterial protease activity in human plasma and wound fluids.

Cai Jun J   Nielsen Maike W MW   Kalogeropoulos Konstantinos K   Auf dem Keller Ulrich U   van der Plas Mariena J A MJA  

iScience 20240126 2

Endogenous and bacterial proteases play important roles in wound healing and infection. Analysis of alterations in the low-molecular-weight peptidome by individual enzymes could therefore provide insight into proteolytic events occurring in wounds and may aid in the discovery of biomarkers. Using liquid chromatography with tandem mass spectrometry, we characterized the peptidome of plasma and acute wound fluids digested <i>ex vivo</i> with human (neutrophil elastase and cathepsin G) and bacteria  ...[more]

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