Proteomics

Dataset Information

0

LC-MS/MS analysis of human brain fibroblast-secreted molecules


ABSTRACT: Our study showed that fibroblasts significantly attenuated blood-brain barrier (BBB) disruption in an in vitro intracerebral hemorrhage (ICH) model. Since fibroblasts and brain microvascular endothelial cells were not in direct contact in this model, we reasoned that fibroblasts did so by secreting molecules with BBB-protective function. Here, we further screened fibroblast-secreted molecules in this in vitro ICH model by mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Fibroblast

DISEASE(S): Intracerebral Hemorrhage

SUBMITTER: Yao Yao  

LAB HEAD: Yao Yao

PROVIDER: PXD037247 | Pride | 2022-11-14

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
F010379.mgf Mgf
F010379.mzid.gz Mzid
F010380.mgf Mgf
F010380.mzid.gz Mzid
F010381.mgf Mgf
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Publications

Fibroblasts repair blood-brain barrier damage and hemorrhagic brain injury via TIMP2.

Xu Lingling L   Nirwane Abhijit A   Xu Ting T   Kang Minkyung M   Devasani Karan K   Yao Yao Y  

Cell reports 20221101 8


The function of fibroblasts in intracerebral hemorrhage (ICH) remains elusive. By targeting Col1α1, a fibroblast-specific marker, we generate mice with ablated Col1α1<sup>+</sup> fibroblasts. These mutants show exacerbated blood-brain barrier (BBB) damage, enlarged injury volume, and worse neurological function, highlighting a beneficial role of Col1α1<sup>+</sup> fibroblasts in ICH. Echoing these findings, fibroblasts significantly decrease endothelial permeability in an in vitro ICH model. Nex  ...[more]

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