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Quantitative proteome and phosphoproteome datasets of DNA replication and mitosis in Saccharomyces cerevisiae

ABSTRACT: Cell division is a highly regulated process that secures the generation of healthy progeny in all organisms, from yeast to human. Dysregulation of this process can lead to uncontrolled cell proliferation and genomic instability, both which are hallmarks of cancer. Cell cycle progression is dictated by a complex network of kinases and phosphatases. These enzymes act on their substrates in a highly specific temporal manner ensuring that the process of cell division is unidirectional and irreversible. Key events of the cell cycle, such as duplication of genetic material and its redistribution to daughter cells, occur in S-phase and mitosis, respectively. Deciphering the dynamics of phosphorylation/dephosphorylation events during these cell cycle phases is important. Here we showcase a quantitative proteomic and phosphoproteomic mass spectrometry dataset that profiles both early and late phosphorylation events and associated proteome alterations that occur during S-phase and mitotic arrest in the model organism S. cerevisiae. This dataset is of broad interest as the molecular mechanisms governing cell cycle progression are conserved throughout evolution.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Joao Paulo

LAB HEAD: Joao A. Paulo

PROVIDER: PXD037291 | Pride | 2022-10-13

REPOSITORIES: Pride

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			Action	DRS
	RossioPaulo_STable1_DiB1.xlsx	Xlsx
	RossioPaulo_STable2_DiB1.xlsx	Xlsx
	RossioPaulo_STable3_DiB1.xlsx	Xlsx
	RossioPaulo_STable4_DiB1.xlsx	Xlsx
	a24533.raw	Raw

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