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Breast cancer cell MCF7+CYP1B1-AS1 LC-MS/MS

ABSTRACT: Long non-coding Rnas (lncRNAs) can act as oncogenes or tumor suppressors to regulate cancer development. We found that CYP1B1-AS1 was down-regulated in breast cancer tissues and correlated with the prognosis of patients. Lentiviral vectors were used to overexpress CYP1B1-AS1 in MCF7 cells, and the target proteins bound to CYP1B1-AS1 were detected by pulldown assay and mass spectrometry. The function of CYP1B1-AS1 is unknown. Our study revealed the molecular mechanism of CYP1B1-AS1 inhibiting breast cancer proliferation in breast cancer, and provided a new strategy for the treatment of breast cancer targeting lncRNA.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: li tang

LAB HEAD: Feng Yan

PROVIDER: PXD037317 | Pride | 2023-10-24

REPOSITORIES: Pride

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			Action	DRS
	QLCJKR076-20210908-P_Proteins.xlsx	Xlsx
	T480_LFQ_CJKR076_PBI_21080008_antisense.raw	Raw
	T480_LFQ_CJKR076_PBI_21080008_sense.raw	Raw

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Publications

FOXO1-regulated lncRNA CYP1B1-AS1 suppresses breast cancer cell proliferation by inhibiting neddylation.

Tang Li L Wei Da D Xu Xinyu X Mo Dongping D Cheng Daofu D Yan Feng F

Breast cancer research and treatment 20230828 2

<h4>Purpose</h4>Overactivated neddylation is considered to be a common event in cancer. Long non-coding RNAs (lncRNAs) can regulate cancer development by mediating post-translational modifications. However, the role of lncRNA in neddylation modification remains unclear.<h4>Methods</h4>LncRNA cytochrome P450 family 1 subfamily B member 1 antisense RNA 1 (CYP1B1-AS1) expression in breast cancer tissues was evaluated by RT-PCR and TCGA BRCA data. Gain and loss of function experiments were performed ...[more]

PMID: 37640964

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