Dataset Information

Quantification of membrane protein expression in G0 and G1 cell cycle phase samples in PC3 prostate cancer cells

ABSTRACT: Prostate cancer (PCa) has a significant ability to disseminate and survive in the bone marrow (BM). Once there, disseminated tumor cells (DTCs) may lie dormant for years, but often eventually reactivate proliferative pathways, thus largely contributing to bone metastasis, disease progression, and relapse. Unfortunately, quiescent PCa cells are difficult to identify and target with treatment, in part because formerly identified relevant markers are intracellular and regulated by protein stability. We sought to address this problem by identifying a G0 marker that is expressed on the cell surface and therefore amenable to antibody binding for identification and therapeutic targeting. In doing so, we utilized stably transduced fluorescent markers for CDKN1B (p27) and CDT1 protein levels, which separate viable PCa cells into G0, G1, or combined S/G2/M populations (as described in PMID 34957090). We then performed FACS to collect G1 and G0 PC3 PCa cells, isolated membrane proteins, and subsequently used GC-MS proteomics to determine differences in protein abundance between G0 and G1. In total, 3,791 proteins were identified.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, Prostate Gland

DISEASE(S): Prostate Adenocarcinoma

SUBMITTER: Henriette Remmer

LAB HEAD: Henriette Remmer

PROVIDER: PXD037378 | Pride | 2023-03-03

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MSB3113G1Band_01.raw	Raw
	MSB3113G1Band_01__F134798_.mzid.gz	Mzid
	MSB3113G1Band_01__F134798_.mzid_MSB3113G1Band_01__F134798_.MGF	Mzid
	MSB3113G1Band_02.raw	Raw
	MSB3113G1Band_02__F134799_.mzid.gz	Mzid

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Publications

HER2 as a potential therapeutic target on quiescent prostate cancer cells.

Yumoto Kenji K Rashid Jibraan J Ibrahim Kristina G KG Zielske Steven P SP Wang Yu Y Omi Maiko M Decker Ann M AM Jung Younghun Y Sun Dan D Remmer Henriette A HA Mishina Yuji Y Buttitta Laura A LA Taichman Russell S RS Cackowski Frank C FC

Translational oncology 20230218

Quiescent prostate cancer (PCa) cells are common in tumors but are often resistant to chemotherapy. Quiescent PCa cells are also enriched for a stem-like tumor initiating population, and can lead to recurrence after dormancy. Unfortunately, quiescent PCa cells are difficult to identify and / or target with treatment in part because the relevant markers are intracellular and regulated by protein stability. We addressed this problem by utilizing PCa cells expressing fluorescent markers for CDKN1B ...[more]

PMID: 36805918

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