Proteomics

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Proteome analysis of the gametophytes of western Himalayan fern Diplazium maximum reveals sucrose inducible proteins of stress tolerance: Insights into the adaptive competence of the fern


ABSTRACT: The proteome of the gametophytes of Diplazium maximum, a temperate Himalayan Polypoidale fern was studied in response to micro-environmental changes. The study was expected to reveal the key proteins associated with a gametophyte’s response to sucrose mediated changes in osmotic potential. A major aim of the study was to identify proteins that would express differentially under micro-environmental stress and also to gain an understanding of the adaptive responses/competence of D. maximum gametophytes. The genes/proteins identified in the study have potential utility in various crop improvement programs.

INSTRUMENT(S): ultraflex

ORGANISM(S): Diplazium Maximum

TISSUE(S): Gametophyte

SUBMITTER: Amita Bhattacharya  

LAB HEAD: Amita Bhattacharya

PROVIDER: PXD014763 | Pride | 2020-05-26

REPOSITORIES: Pride

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Publications

Proteome Analysis of the Gametophytes of a Western Himalayan Fern <i>Diplazium maximum</i> Reveals Their Adaptive Responses to Changes in Their Micro-Environment.

Sareen Bhuvnesh B   Thapa Pooja P   Joshi Robin R   Bhattacharya Amita A  

Frontiers in plant science 20191217


Ferns have survived changing habitats and environmental extremes of different eras, wherein, the exploratory haploid gametophytes are believed to have played a major role. Therefore, the proteome of <i>in vitro</i> grown gametophytes of a temperate Himalayan fern, <i>Diplazium maximum</i> in response to 0 (G0), 1 (G1), and 3% (G3) sucrose was studied. A total of 110 differentially abundant protein spots (DAPs) were obtained. Among these, only 67 could be functionally categorized as unique protei  ...[more]

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