Dataset Information

Inhibitors molecules isolated from midgut and midgut diverticula of the spider Nephilingis cruentata

ABSTRACT: Spiders are insect predators and their venoms are important to prey capture. Spider venomshave several potential applications as pharmaceutical compounds and insecticides. However,transcriptomic and proteomic analyses of the digestive system (DS) of spiders show that DS is also a rich source of new inhibitor molecules. Biochemical, transcriptomic and proteomic data of crude DS extracts show the presence of molecules with inhibitor potential in the spider Nephilingis cruentata. Therefore, the aims of this work were to isolate and characterize molecules with serine peptidase inhibitory activity. The DS of fasting adult females was homogenized under acidic conditions and subjected to heat treatment. After that, samples were submitted to ion exchange batch and high-performance reverse-phase chromatography. The fractions with inhibitory activity were confirmed by mass spectrometry, identifying 6 molecules with inhibitor potential. The inhibitor Nc6834 was kinetically characterized, showing a KD value of 30.25 nM ± 8.13. Analysis of the tertiary structure by molecular modeling using Alpha-Fold2 indicates that the inhibitor Nc6834 structurally belongs to theMIT1-like atracotoxin family. This is the first time that a serine peptidase inhibitory function is attributed to this structural family and the reactive site inhibitor residue is identified. Sequence analysis indicates that these molecules may be present in the DS of other spiders.

INSTRUMENT(S): Dionex instrument model

ORGANISM(S): Nephilengys Cruentata

TISSUE(S): Epithelium Of Midgut, Midgut Diverticulum

SUBMITTER: Oscar Bento

LAB HEAD: Adriana Rios Lopes

PROVIDER: PXD037594 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Nc6834-P4-05_RA6_01_1010.mzML	Mzml
	Nc6834-P4-05_RA6_01_1010.mzxml	Mzxml
	Oscar_nc6834-P4_-05_RA6_01_1010.mgf	Mgf
	checksum.txt	Txt
	peptides_1_1_0.mzid.gz	Mzid

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Publications

Spiders' digestive system as a source of trypsin inhibitors: functional activity of a member of atracotoxin structural family.

Neto Oscar Bento Silva OBS Valladão Rodrigo R Coelho Guilherme Rabelo GR Dias Renata R Pimenta Daniel Carvalho DC Lopes Adriana Rios AR

Scientific reports 20230210 1

Spiders are important predators of insects and their venoms play an essential role in prey capture. Spider venoms have several potential applications as pharmaceutical compounds and insecticides. However, transcriptomic and proteomic analyses of the digestive system (DS) of spiders show that DS is also a rich source of new peptidase inhibitor molecules. Biochemical, transcriptomic and proteomic data of crude DS extracts show the presence of molecules with peptidase inhibitor potential in the spi ...[more]

PMID: 36765114

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