Dataset Information

IDA-SWATH proteomic analysis of rat hippocampus upon methylglyoxal treatment

ABSTRACT: Diabetes is one of the major risk factors for Alzheimer’s disease (AD) development. The role of elevated levels of glucose, methylglyoxal (MGO), and advanced glycation end products (AGEs) in diabetes in the pathogenesis of the AD is not well understood. In this pursuit, we studied the role of methylglyoxal in the pathogenesis of AD in rat models. The elevated plus-maze (EPM) behavioural study indicated that MGO induces anxiety. Treatment of telmisartan (RAGE expression inhibitor) and aminoguanidine (MGO quencher) attenuated MGO induced anxiety. Further, hippocampal proteomics demonstrated that MGO treated rats differentially regulate proteins involved in calcium homeostasis, mitochondrial functioning, and apoptosis which may affect neurotransmission and neuronal plasticity. Hippocampal tau phosphorylation level was increased in MGO treated rats which was reduced in presence in aminoguanidine and telmisartan. Plasma fructosamine level was increased upon MGO treatment. Hippocampal histochemistry showed vascular degeneration and neuronal loss upon MGO treatment. This study provides mechanistic insight into the role of MGO in the diabetes-associated development of AD.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Gouri Patil

LAB HEAD: Mahesh Kulkarni

PROVIDER: PXD037673 | Pride | 2024-01-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	C_STZ_MGO_MGOAMG_MGOTELMI_12th_dec_2019.group	Other
	C_STZ_MGO_MGOAMG_MGOTELMI_12th_dec_2019_MGF.mgf	Mgf
	C_STZ_MGO_MGOAMG_MGOTELMI_12th_dec_2019_MZID.mzid	Mzid
	Control_B1_IDA.wiff	Wiff
	Control_B1_IDA.wiff.scan	Wiff

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Publications

Behavioral and Proteomic Studies Reveal Methylglyoxal Activate Pathways Associated with Alzheimer's Disease.

Patil Gouri G Kulsange Shabda S Kazi Rubina R Chirmade Tejas T Kale Vaikhari V Mote Chandrashekhar C Aswar Manoj M Koratkar Santosh S Agawane Sachin S Kulkarni Mahesh M

ACS pharmacology & translational science 20221227 1

Diabetes is one of the major risk factors for Alzheimer's disease (AD) development. The role of elevated levels of glucose, methylglyoxal (MGO), and advanced glycation end products (AGEs) in the pathogenesis of AD is not well understood. In this pursuit, we studied the role of methylglyoxal in the pathogenesis of AD in rat models. The elevated plus-maze (EPM) behavioral study indicated that MGO induces anxiety. Treatment of telmisartan (RAGE expression inhibitor) and aminoguanidine (MGO quencher ...[more]

PMID: 36654748

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