Dataset Information

An Optimized Suspension Trapping (S-Trap) Method for Phosphoproteomics Sample Preparation

ABSTRACT: The performance of the S-Trap protocol for phosphoproteomics studies for proteome and phosphoproteome was systematically evaluated in big-scale and small-scale samples. The results showed the existing protocol was problematic for phsophopeptides enrichment. After employing optimized S-Trap methodology, more peptides and phosphopeptides are found in the application for extracellular vesicles.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Fujia Wang

LAB HEAD: Maarten Altelaar

PROVIDER: PXD037751 | Pride | 2023-06-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Big-scale_phosphoproteome_RAW.zip	Other
	Big-scale_proteome_RAW.zip	Other
	EV_Raw.zip	Other
	Samll-scale_phosphoproteome_RAW.zip	Other
	Small-scale_proteome_RAW.zip	Other

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Publications

Optimized Suspension Trapping Method for Phosphoproteomics Sample Preparation.

Wang Fujia F Veth Tim T Kuipers Marije M Altelaar Maarten M Stecker Kelly E KE

Analytical chemistry 20230615 25

A successful mass spectrometry-based phosphoproteomics analysis relies on effective sample preparation strategies. Suspension trapping (S-Trap) is a novel, rapid, and universal method of sample preparation that is increasingly applied in bottom-up proteomics studies. However, the performance of the S-Trap protocol for phosphoproteomics studies is unclear. In the existing S-Trap protocol, the addition of phosphoric acid (PA) and methanol buffer creates a fine protein suspension to capture protein ...[more]

PMID: 37319171

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