Dataset Information

Arabidopsis DMC1 ubiquitination sites identification, LC-MS/MS

ABSTRACT: To characterize the mechanism of DMC1 ubiquitination as well as further explore the impact of DMC1’s ubiquitination on meiosis, we attempted to identify the potential DMC1 ubiquitination sites. The in vitro ubiquitination assay using recombinant DMC1 heterologously expressed and purified from E. coli as substrate was conducted. Then mass spectrometry assay was performed to identify the ubiquitination sites of DMC1. Five DMC1 lysine residues (K45, K70, K101, K162, and K290) were identified as potential ubiquitination sites.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell

SUBMITTER: Wanyue Xu

LAB HEAD: Yingxiang Wang

PROVIDER: PXD038116 | Pride | 2023-10-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Arabidopsis_DMC1_in_vitro_ubiquitination_assay-MS-1.mgf	Mgf
	Arabidopsis_DMC1_in_vitro_ubiquitination_assay-MS-1.msf	Msf
	Arabidopsis_DMC1_in_vitro_ubiquitination_assay-MS-1.raw	Raw
	Arabidopsis_DMC1_in_vitro_ubiquitination_assay-MS-1.xml	Xml
	Arabidopsis_DMC1_in_vitro_ubiquitination_assay-MS-3.mgf	Mgf

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Publications

SCF<sup>RMF</sup> mediates degradation of the meiosis-specific recombinase DMC1.

Xu Wanyue W Yu Yue Y Jing Juli J Wu Zhen Z Zhang Xumin X You Chenjiang C Ma Hong H Copenhaver Gregory P GP He Yan Y Wang Yingxiang Y Wang Yingxiang Y

Nature communications 20230819 1

Meiotic recombination requires the specific RecA homolog DMC1 recombinase to stabilize strand exchange intermediates in most eukaryotes. Normal DMC1 levels are crucial for its function, yet the regulatory mechanisms of DMC1 stability are unknown in any organism. Here, we show that the degradation of Arabidopsis DMC1 by the 26S proteasome depends on F-box proteins RMF1/2-mediated ubiquitination. Furthermore, RMF1/2 interact with the Skp1 ortholog ASK1 to form the ubiquitin ligase complex SCF<sup> ...[more]

PMID: 37598222

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