Proteomics

Dataset Information

0

Phosphoproteomic and proteomic analysis of early epileptogenesis in mice at 4 and 24 h post-status epilepticus


ABSTRACT: The phosphorylation-based signalling and protein changes occurring in the early phases after a pathophysiological insult, like status epilepticus, have not been detailed. To this end, the hippocampi of mice treated with pilocarpine and diazepam were examined by mass spectrometry at 4 and 24 h post-status epilepticus at vast depth using the tandem mass tag 11-plex system. In the accompanying article, the results implicated posttranscriptional regulatory proteins as early targets of increased phosphorylation. Also, the major targets of decreased phosphorylation at 4 h and 24 h were a subset of post synaptic density scaffold proteins, ion channels and neurotransmitter receptors. Many proteins targeted by dephosphorylation at 4 h also had decreased protein abundance at 24 h, indicating a phosphatase-mediated weakening of synapses.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Hippocampus

DISEASE(S): Epilepsy

SUBMITTER: Mark Graham  

LAB HEAD: Mark Evan Graham

PROVIDER: PXD038241 | Pride | 2024-02-22

REPOSITORIES: Pride

altmetric image

Publications

Multiomics of early epileptogenesis in mice reveals phosphorylation and dephosphorylation-directed growth and synaptic weakening.

Hurtado Silva Mariella M   van Waardenberg Ashley J AJ   Mostafa Aya A   Schoch Susanne S   Dietrich Dirk D   Graham Mark E ME  

iScience 20240319 4


To investigate the phosphorylation-based signaling and protein changes occurring early in epileptogenesis, the hippocampi of mice treated with pilocarpine were examined by quantitative mass spectrometry at 4 and 24 h post-status epilepticus at vast depth. Hundreds of posttranscriptional regulatory proteins were the major early targets of increased phosphorylation. At 24 h, many protein level changes were detected and the phosphoproteome continued to be perturbed. The major targets of decreased p  ...[more]

Shared Molecules

Only show the datasets with similarity scores above: 0.5
     

Similar Datasets

2019-05-20 | PXD010242 | Pride
2022-02-14 | PXD016636 | Pride
2019-07-22 | PXD010452 | Pride
2019-11-06 | PXD014076 | Pride
2012-05-02 | E-GEOD-33648 | biostudies-arrayexpress
2021-11-02 | PXD014443 | Pride
2017-05-16 | PXD005311 | Pride
2022-06-13 | E-MTAB-11093 | biostudies-arrayexpress
2024-10-08 | E-MTAB-14500 | biostudies-arrayexpress
2024-04-23 | PXD047423 | Pride