Proteomics

Dataset Information

0

Proteomics and phosphoproteomics of the diurnal cycle in chow and high fat high sucrose feed rats

ABSTRACT: There is growing evidence that energy metabolism and insulin action are regulated by mechanisms that follow a diurnal rhythm and it has been proposed that defects in Akt signalling are associated with the pathophysiology of metabolic disease. It is therefore important to investigate these parameters under physiology of the free-living state. We therefore examined the insulin action in muscle of chow or high fat, high sucrose diet-fed (HFHS) rats during the normal diurnal cycle. HFHS animals displayed hyperinsulinemia, however had reduced systemic glucose disposal and impaired muscle glucose uptake during the feeding period. Proteomics and phosphoproteomics was performed over the diurnal cycle in chow and HFHS rats.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Skeletal Muscle

SUBMITTER: Benjamin Parker  

LAB HEAD: Benjamin Parker

PROVIDER: PXD010242 | Pride | 2019-05-20

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20170609_QEplus_BP_LS_Circadian_TMT1_mono_1.raw Raw
20170609_QEplus_BP_LS_Circadian_TMT1_mono_10.raw Raw
20170609_QEplus_BP_LS_Circadian_TMT1_mono_11.raw Raw
20170609_QEplus_BP_LS_Circadian_TMT1_mono_12.raw Raw
20170609_QEplus_BP_LS_Circadian_TMT1_mono_2.raw Raw
Items per page:
1 - 5 of 78
altmetric image

Publications

Reduced insulin action in muscle of high fat diet rats over the diurnal cycle is not associated with defective insulin signaling.

Small Lewin L   Brandon Amanda E AE   Parker Benjamin L BL   Deshpande Vinita V   Samsudeen Azrah F AF   Kowalski Greg M GM   Reznick Jane J   Wilks Donna L DL   Preston Elaine E   Bruce Clinton R CR   James David E DE   Turner Nigel N   Cooney Gregory J GJ  

Molecular metabolism 20190412

<h4>Objective</h4>Energy metabolism and insulin action follow a diurnal rhythm. It is therefore important that investigations into dysregulation of these pathways are relevant to the physiology of this diurnal rhythm.<h4>Methods</h4>We examined glucose uptake, markers of insulin action, and the phosphorylation of insulin signaling intermediates in muscle of chow and high fat, high sucrose (HFHS) diet-fed rats over the normal diurnal cycle.<h4>Results</h4>HFHS animals displayed hyperinsulinemia b  ...[more]

Similar Datasets

Proteomics Interferon stimulated changes in the brain phosphoproteome
2021-11-02 | PXD014443 | Pride
Proteomics Phosphoproteomic and proteomic analysis of early epileptogenesis in mice at 4 and 24 h post-status epilepticus
2024-02-22 | PXD038241 | Pride
Proteomics Phosphoproteomic analysis of mouse neurons with SRPK2 overexpression or knockdown
2022-02-14 | PXD016636 | Pride
Transcriptomics Genome wide rhythmic transcription under light/dark cycles in Ostreococcus tauri
2010-04-20 | E-GEOD-16422 | biostudies-arrayexpress
Proteomics Phosphoproteomics of rodent exercise models
2019-07-22 | PXD010452 | Pride
Proteomics The phosphoproteome of the coral model system Aiptasia
2019-11-06 | PXD014076 | Pride
Proteomics The molecular landscape of homologous recombination deficient, end-stage, high grade serous ovarian cancer
2023-02-27 | PXD030034 | Pride
Transcriptomics Expression Analysis of Trimethyltin Chlorides (TMT) Impact on Embryonic Development
2011-03-23 | E-GEOD-28131 | biostudies-arrayexpress
Transcriptomics Transcription profiling of rat at pheochromocytoma cell line PC12 treated with trimethyltin at low and high doses
2009-04-29 | E-GEOD-5073 | biostudies-arrayexpress
Proteomics Arabidopsis diurnal LFQ proteome and labelled phosphoproteome
2020-12-18 | PXD007600 | Pride